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Si0265877 hs ptprj 6

Manufactured by Qiagen
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The SI0265877 HS_PTPRJ_6 is a laboratory equipment product manufactured by Qiagen. The core function of this product is to facilitate the silencing of the PTPRJ gene, which encodes for the protein tyrosine phosphatase receptor type J. This product is designed for use in research applications.

Automatically generated - may contain errors

Lab products found in correlation

Lipofectamine rnaimax, by Thermo Fisher Scientific (2 mentions) Opti mem, by Thermo Fisher Scientific (2 mentions) Ptp1b, by Merck Group (2 mentions) Sr321721 c, by Merck Group (2 mentions) Veptp, by Merck Group (2 mentions) Sasi hs02 00324728, by Merck Group (2 mentions)

2 protocols using si0265877 hs ptprj 6

1

Knockdown of Signaling Regulators in HUVEC

Check if the same lab product or an alternative is used in the 5 most similar protocols
HUVEC were seeded onto 6-well plates and transfected at 80% confluency with 2.5 ml Opti-MEM (Thermo Fisher) with indicated siRNA plus 2.5 μL Lipofectamine RNA iMAX (Thermo Fisher) for 6–8 hrs. Transfection mix was replaced with full media (EGM-2 MV) for 48 hours, then cell starved in 2% FBS for 6–8 hrs before growth factor stimulation. Following siRNA were used for knockdown experiments in HUVEC: Sdc2 (Origene #SR321721 – C, 40 nM), VEPTP (Sigma-Aldrich SASI_Hs02_00324728, 60 nM), PTP1B Sigma-Aldrich SAS1_Hs01_00230699, 60nM) DEP1 (Qiagen #SI0265877 HS_PTPRJ_6, 60 nM)
Corti F., Ristori E., Rivera-Molina F., Toomre D., Zhang J., Mihailovic J., Zhuang Z, & Simons M. (2022). Syndecan-2 selectively regulates VEGF-induced vascular permeability. Nature cardiovascular research, 1(5), 518-528.
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2

Knockdown of Signaling Regulators in HUVEC

Check if the same lab product or an alternative is used in the 5 most similar protocols
HUVEC were seeded onto 6-well plates and transfected at 80% confluency with 2.5 ml Opti-MEM (Thermo Fisher) with indicated siRNA plus 2.5 μL Lipofectamine RNA iMAX (Thermo Fisher) for 6–8 hrs. Transfection mix was replaced with full media (EGM-2 MV) for 48 hours, then cell starved in 2% FBS for 6–8 hrs before growth factor stimulation. Following siRNA were used for knockdown experiments in HUVEC: Sdc2 (Origene #SR321721 – C, 40 nM), VEPTP (Sigma-Aldrich SASI_Hs02_00324728, 60 nM), PTP1B Sigma-Aldrich SAS1_Hs01_00230699, 60nM) DEP1 (Qiagen #SI0265877 HS_PTPRJ_6, 60 nM)
Corti F., Ristori E., Rivera-Molina F., Toomre D., Zhang J., Mihailovic J., Zhuang Z, & Simons M. (2022). Syndecan-2 selectively regulates VEGF-induced vascular permeability. Nature cardiovascular research, 1(5), 518-528.
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