Total RNAs were extracted from S. miltiorrhiza seedlings and treated with RNase-free DNase I (Foregene CO., LTD., Chengdu, China). And Validation using the same RNA samples as for RNA-seq sequencing. The reverse transcription reaction is performed using 800 ng of the total RNA samples with RT Easy™II Kit (Foregene CO., LTD., Chengdu, China). The cDNA products were used as templates for quantitative PCR. The qPCR reaction was carried out with the Real-Time PCR Easy™-SYBR Green I (Foregene CO., LTD., Chengdu, China) in an FQD-48A Real-Time PCR System (Bioer, China). All the primers used for RT-qPCR are designed on Primer 6 software and listed in Table S1. The SmActin gene was used as the internal control, and the 2−△△CT method calculated the relative expression levels29 (link). Three biological replications and technical replications were implemented for each sample to ensure reproducibility and reliability.
Rt easy 2 kit
Manufactured by Foregene
Sourced in China
The RT Easy™II Kit is a laboratory equipment product designed for reverse transcription. It converts RNA into complementary DNA (cDNA) for use in downstream applications. The kit includes necessary reagents and buffers to perform the reverse transcription reaction.
Automatically generated - may contain errors
3 protocols using rt easy 2 kit
1
Transcriptome Analysis of S. miltiorrhiza
2
RNA Extraction and qPCR Analysis
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Total RNA was extracted using TRIGene (Genstar). 1 μg of RNA was reverse transcript using the RT Easy II kit (Foregene). qPCR was performed using SYBR Green Master Mix (Yeasen), with GAPDH as the reference gene. Primer sequences are in Additional file 1 : Table S2.
3
Total RNA Isolation and cDNA Synthesis from S. miltiorrhiza
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Total RNA from S. miltiorrhiza samples were isolated using the Plant Total RNA Isolation Kit Plus following the manufacturer’s protocol (Foregene, Ltd., Chengdu, China). cDNA synthesis was done by using 1 ug of the total RNA samples with RT Easy™ II Kit (Foregene, Ltd., Chengdu, China).
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!