The carbonyl compounds in the working standards and sample solutions were analyzed using an HPLC system equipped with a UV detector (20A Series, Shimadzu, Kyoto, Japan). The working standards and sample solutions were placed in the HPLC autosampler and automatically injected into the HPLC column. The carbonyl compounds were separated on a Shim-Pack GIS-ODS column (length = 250 mm, diameter = 4.6 mm, and particle size = 5 μm) using a mobile phase of acetonitrile and distilled water at a flow rate of 1.5 mL·min−1 (total run time = 15 min). The separated compounds were detected using a UV detector at a wavelength of 360 nm. The detailed conditions are presented in Table 7 .
Gis ods column
Manufactured by Shimadzu
The GIS-ODS column is a specialized high-performance liquid chromatography (HPLC) column designed for the separation and analysis of organic compounds. It features an octadecylsilyl (ODS) stationary phase, which is commonly used for reversed-phase chromatography. The core function of this column is to facilitate the separation and detection of a wide range of organic molecules in complex samples.
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2 protocols using gis ods column
1
Quantitative Analysis of Carbonyl Compounds
2
HPLC-UV Analysis of Aldehydes
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All liquid samples (DNPH cartridge solvent extraction samples and working standards) containing three aldehydes were analyzed with an HPLC-UV system (LC-2010, Shimadzu, Japan) equipped with an auto sampler (SIL-20A), pump (LC-20AD), oven (CTO-20A), and UV detector (SPD-20A). A fixed sample volume of 20 μL was injected into the HPLC system through the auto sampler. The analytes were separated on a Shim-Pack GIS-ODS column (length: 25 mm, diameter: 4.6 mm, particle size: 5 μL) using a mobile phase of acetonitrile–distilled water (6:4 (v/v)) at a flow rate of 1.5 mL min–1 at 30 °C (maintained by the oven). The total run time was 11 min. The separated aldehydes were detected by the UV detector at a wavelength of 360 nm (Table S2 ).
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