Uas ser

Flies were raised at 25°C, on regular fly food. Sik2^Δ41 and UAS-Sik2^K170M lines were kindly provided by Jongkyeong Chung (Seoul, Korea) [9 (link)]. Sensitized and eyeful lines were kindly provided by Bassem Hassan (Paris, France) [2 (link)]. UAS-Dicer2; ey-GAL4, lGMR-Gal4 / CyO was a kind gift from Claude Desplan (New York, USA) [40 (link)].
The following fly lines were obtained from the Bloomington Drosophila Stock Center: w¹¹¹⁸ (3605), UAS-Notch (26820), UAS-Delta (5612), UAS-Ser (5815), UAS-Fringe (5831), UAS-Delta.DN (26697), lGMR-GAL4 (8121), ey-GAL4 (5535), elav-GAL4 (458), mirr-GAL4 (29650), sca-GAL4 (6479), neoFRT42D, GMR-myr-GFP (7110), neoFRT42D, GMR-hid / CyO; ey-GAL4, UAS-FLP (5251), actin-GAL4 (25374). Following RNAi lines were supplied by the VDRC Stock Center: Sik3-RNAi (107458), Sik2-RNAi (103739), Ser-RNAi (108348), Delta-RNAi (3720), Fringe-RNAi (51977).

Overexpression studies were performed using the Gal4/UAS system^{36 (link)}. We used the following UAS lines: UAS-Dl, UAS-Ser, UAS-mCD8-ChRFP (Bloomington Drosophila Stock Center; BDSC), UAS-fwdIR, UAS-sktlIR, UAS-plc-21IR, UAS-dgkIR, UAS-gfpIR (Vienna Drosophila RNAi Center). UAS-pld38, and UAS-rdgA/dgk were a generous gift from Padinjat Raghu (National Centre for Biological Sciences, Tata Institute of Fundamental Research, India). We also used the following Gal4 lines: pnr-Gal4, en-Gal4 and sca-Gal4 (BDSC). We also used the following strains Dl^[Rev10], Ser^[RX82], Spdo^G104, Ap-2α^[40–31], numb^{1537 (link)}. SpdoCh2GFP3, neur-nlsFP670, NiGFP were a generous gift from François Schweisguth (Institut Pasteur, France). UAS-lipinwt was a generous gift from Michael Lehmann (University of Arkansas, USA). All phenotypes were analysed at 25 °C unless stated otherwise.