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Quantikine human

Manufactured by R&D Systems
Sourced in United States

The Quantikine Human is a quantitative sandwich enzyme-linked immunosorbent assay (ELISA) designed for the measurement of specific human proteins in cell culture supernates, serum, plasma, and other biological fluids.

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2 protocols using quantikine human

1

Measurement of Inflammatory Biomarkers

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Serum samples were obtained after 8–10 h overnight fasting, and frozen immediately at −80°C. Routine biochemical parameters were measured using standard methods. Serum high sensitive C-reactive protein (hs-CRP) was measured by an immunoturbidimetric fully automated assay (Roche Diagnostics GmbH, Mannheim, Germany) with functional sensitivity of 0.3 mg/L (intra- and inter-assay precision, 1.6 and 8.4, resp.). Serum levels of tumor necrosis factor-α (TNF-α), interleukin (IL)-6, and IL-10 were determined by a high-sensitive ELISA method (Quantikine Human, R&D Systems, Minneapolis, USA) in a DSXTM 4 Plate ELISA Processor (Vitro SA, Spain). Minimum detectable concentrations were 0.10 pg/mL, 0.70 pg/mL, and 0.50 pg/mL, respectively. Intra- and inter-assay coefficients of variation (CV) were <10.8% in all cases.
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2

Quantifying IGF-1 and IGFBP3 Levels

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IGF-1 and IGFBP3 were evaluated by a commercially available sandwich immunoassay (Quantikine human, R&D Systems, Minneapolis, MN).
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