c-Myc inhibitors (10,058-F4 and 10,074-G5) were obtained from Selleckchem (Houston, TX, USA). Artemisinin (ARTS), Dihydroartemisinin (DHA), Shikonin, Oridonin, Andrographolide, Baicalein, Sulforaphane and Luteolin (purity ≥98%) were purchased from Shanghai Yuanye Bio-Technology Co., Ltd. (Shanghai, China). RPMI-1640, Dulbecco’s Modified Eagle’s medium (DMEM), Opti-MEM and fetal bovine serum (FBS) were purchased from Gibco/BRL (Grand Island, NY, USA). Sulforhodamine B (SRB) and DMSO were purchased from Sigma-Aldrich (St. Louis, MO, USA). Homo c-Myc plasmid was obtained from Gene Pharma (Gene Pharma Co., Ltd., China). Primary antibodies against c-Myc and PD-L1 were purchased from Cell Signaling Technology (Beverly, MA, USA); β-actin was purchased from Santa Cruz Biotechnology (Santa Cruz, CA, USA). All secondary antibodies were purchased from Cell Signaling Technology (Beverly, MA, USA).
Andrographolide
Manufactured by Yuanye Bio-Technology
Sourced in China
Andrographolide is a bioactive compound extracted from the plant Andrographis paniculata. It is a white, crystalline solid with a molecular formula of C20H30O5. Andrographolide is known for its potential anti-inflammatory, antioxidant, and immunomodulatory properties. While its specific functions are an active area of research, this product is intended for further scientific investigation and should not be interpreted or extrapolated beyond its core characteristics.
Automatically generated - may contain errors
Lab products found in correlation
Dihydroartemisinin, by Yuanye Bio-Technology (2 mentions)
Dimethyl sulfoxide (dmso), by Merck Group (2 mentions)
β actin, by Santa Cruz Biotechnology (2 mentions)
Baicalein, by Yuanye Bio-Technology (1 mentions)
Oridonin, by Yuanye Bio-Technology (1 mentions)
Dulbecco s modified eagle s medium dmem, by Thermo Fisher Scientific (1 mentions)
Sulforaphane, by Yuanye Bio-Technology (1 mentions)
Sulforhodamine b srb, by Merck Group (1 mentions)
Shikonin, by Yuanye Bio-Technology (1 mentions)
Rpmi 1640, by Thermo Fisher Scientific (1 mentions)
Fetal bovine serum (fbs), by Thermo Fisher Scientific (1 mentions)
Opti mem, by Thermo Fisher Scientific (1 mentions)
Luteolin, by Yuanye Bio-Technology (1 mentions)
N cadherin, by Boster Bio (1 mentions)
Vimentin, by Boster Bio (1 mentions)
P mtor, by Santa Cruz Biotechnology (1 mentions)
Antibody against lc3b, by Abcam (1 mentions)
P jnk, by Beyotime (1 mentions)
Snail, by Santa Cruz Biotechnology (1 mentions)
P akt, by Beyotime (1 mentions)
3 protocols using andrographolide
1
Inhibiting c-Myc Signaling Pathways
2
Andrographolide Modulates PI3K Signaling
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Andrographolide was purchased from Shanghai Yuanye Bio-Technology (Shanghai, China) and dissolved in DMSO (Sigma-Aldrich Chemical Co., St. Louis, MO, USA) to obtain a stock solution of 100 mM. Insulin-like growth factor 1 (IGF-1, a PI3K activator) was purchased from Puxin Bio (Shanghai, China), and LY294002 (a PI3K inhibitor) was purchased from AbMole China (Shanghai, China).
3
Andrographolide and Dihydroartemisinin Signaling
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Andrographolide and Dihydroartemisinin was purchased from Yuanye Biotechnology Co. Ltd (Shanghai,China). PI3K Class I, PI3K Class III E-cadherin, Snail, p-mTOR, mTOR, p-ERK, ERK and β-actin antibodies were purchased from Santa cruz Biotechnology (Santa cruz, CA, USA). Vimentin, N-cadherin, Atg5 and Beclin-1 antibodies were obtained from Boster Biological Technology Co. Ltd (Wuhan, China). U0126, SP600125, p-Akt, Akt, p-JNK, JNK, p-p38 and p38 antibodies were obtained from Beyotime Institute of Biotechnology (Haimen, China). Antibody against LC3B was purchased from Abcam (Cambridge, UK). All other reagents were from common commercial sources.
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