Crystal violet, sodium dodecyl sulfate (SDS), bovine serum albumin (BSA), insulin, Triton X-100, trypsin, cordycepin, and trypan blue were purchased from Sigma Chemical Co. (St. Louis, MO, USA). Moreover, fetal bovine serum (FBS) was purchased from Life Technologies (Auckland, New Zealand), and dimethyl sulfoxide was purchased from Wako Pure Chemical Industries (Saitama, Japan). Vimentin antibody, E-cadherin antibody, and GAPDH antibodies were purchased from Santa Cruz (Santa Cruz, CA, USA). The PGC-1alpha antibody was obtained from Novus (Littleton, CO, USA). TGF-beta was purchased from Preprotech (London, UK). CD44-FITC antibody and CD117-PE antibody were purchased from (eBioscience, CA, USA). Epidermal growth factor and fibroblast growth factor were purchased from Invitrogen (CA, USA).
Cd44 fitc antibody
Manufactured by Thermo Fisher Scientific
Sourced in United States
The CD44-FITC antibody is a fluorescently labeled monoclonal antibody that binds to the CD44 cell surface glycoprotein. CD44 is a cell adhesion molecule involved in various cellular processes. The FITC (fluorescein isothiocyanate) label allows for the detection and analysis of CD44-expressing cells using flow cytometry or other fluorescence-based techniques.
Automatically generated - may contain errors
Lab products found in correlation
Triton x 100, by Merck Group (1 mentions)
Crystal violet, by Merck Group (1 mentions)
E cadherin antibody, by Santa Cruz Biotechnology (1 mentions)
Gapdh antibody, by Santa Cruz Biotechnology (1 mentions)
Fetal bovine serum (fbs), by Thermo Fisher Scientific (1 mentions)
Bovine serum albumin (bsa), by Merck Group (1 mentions)
Epidermal growth factor (egf), by Thermo Fisher Scientific (1 mentions)
Trypsin, by Merck Group (1 mentions)
Fibroblast growth factor (fgf), by Thermo Fisher Scientific (1 mentions)
Insulin, by Merck Group (1 mentions)
Trypan blue, by Merck Group (1 mentions)
Dimethyl sulfoxide (dmso), by Fujifilm (1 mentions)
Vimentin antibody, by Santa Cruz Biotechnology (1 mentions)
Cordycepin, by Merck Group (1 mentions)
Trypsin edta, by Thermo Fisher Scientific (1 mentions)
Facsdiva, by BD (1 mentions)
2 protocols using cd44 fitc antibody
1
Comprehensive Reagent Procurement for Cell Culture
2
Immunophenotyping of Bone Marrow Stromal Cells
Check if the same lab product or an alternative is used in the 5 most similar protocols
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The expressions of cell surface markers on BMSCs were evaluated by ow cytometry. BMSCs were detached from the culture dish using 0.05% trypsin-EDTA (Gibco), centrifuged, rinsed and re-suspended in PBS at a concentration of 10 5 cells/mL. Re-suspended cells were incubated with 5 mL of CD44-FITC antibody (eBioscience, San Diego, CA, USA), CD45-BV510 antibody (eBioscience), CD29-APC antibody (eBioscience) and CD105-PE antibody (eBioscience) in the dark at 4 ℃ for 30 min. After being washed twice with PBS, cytometric analysis was performed was performed with FACSDiva (Canto, BD Bioscience, San Jose, CA, USA) and data were analyzed with FlowJo software (Tree Star, Ashland, OR, USA) (Supplement gure).
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