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Fitc conjugated rat anti mouse igg1 and igg2a 2b

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The FITC)-conjugated rat anti-mouse IgG1 and IgG2a/2b is a laboratory reagent used for the detection and identification of mouse immunoglobulin subclasses IgG1 and IgG2a/2b. The reagent is conjugated with the fluorescent dye fluorescein isothiocyanate (FITC), which allows for the visualization and analysis of the target immunoglobulins in various immunological techniques.

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Close spelling variants of this product

FITC-conjugated Rat IgG2a (2 citations) FITC-conjugated rat IgG1 (2 citations) IgG2a/2b‐FITC (2 citations) Anti-rat IgG2a-FITC (2 citations) FITC-conjugated mouse IgG2a (3 citations) FITC-conjugated mouse IgG1 (7 citations) IgG1 and IgG2a (4 citations) Rat anti-mouse IgG2a (6 citations) FITC anti-mouse IgG2a (2 citations) FITC-conjugated anti-IgG1 (2 citations)

2 protocols using fitc conjugated rat anti mouse igg1 and igg2a 2b

1

Detecting Alloantibodies Post-BMT

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Recipient serum harvested >3 months post-BMT was heat-inactivated and incubated with recipient-type, donor-type and third-party-type thymocytes (which are low in Fc receptors, reducing background staining). Binding of serum IgG antibodies to thymocytes was analyzed by flow cytometry using fluorescein isothiocyanate (FITC)-conjugated rat anti-mouse IgG1 and IgG2a/2b (BD Pharmingen).
Pilat N., Farkas A.M., Mahr B., Schwarz C., Unger L., Hock K., Oberhuber R., Aumayr K., Wrba F, & Wekerle T. (2014). T-regulatory cell treatment prevents chronic rejection of heart allografts in a murine mixed chimerism model. The Journal of Heart and Lung Transplantation, 33(4), 429-437.
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2

Measurement of Recipient Serum IgG Binding

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Recipient serum harvested >3 months after BMT was heat-inactivated and incubated with recipient-type and donor-type thymocytes. Binding of serum IgG Abs to thymocytes was analyzed by flow cytometry using FITC-conjugated rat anti-mouse IgG1 and IgG2a/2b (BD Pharmingen).
Pilat N., Klaus C., Hock K., Baranyi U., Unger L., Mahr B., Farkas A.M., Wrba F, & Wekerle T. (2015). Polyclonal Recipient nTregs Are Superior to Donor or Third-Party Tregs in the Induction of Transplantation Tolerance. Journal of Immunology Research, 2015, 562935.
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