Antibodies used were: rabbit monoclonal anti-Src (#32G6, Cell Signaling Technologies, Danvers, MA) diluted 1:5000; rabbit polyclonal anti-Pyk2 (#3292, Cell Signaling Technologies, Danvers, MA) diluted 1:2000; Rabbit polyclonal anti-MBD2 (#ab224827, Abcam, Cambridge, MA diluted 1:1000; 3 additional MBD2 antibodies were used in (unsuccessful) efforts to detect FP-MBD2 including: rabbit anti-MBD2 (#NB100-81657, Novus Biologicals, Centennial, CO); mouse monoclonal anti-MBD2 (#N2C3, GeneTex, Irvine, CA); and rabbit polyclonal anti-MBD2 (#ab38646, Abcam, Cambridge, MA). GFP was detected using mouse monoclonal anti-GFP (Living Colors GFP monoclonal antibody, Clontech, Mountain View, CA) diluted 1:10,000; HRP-goat-anti-mouse IgG antibody or HRP rabbit-anti-mouse IgG (Jackson ImmunoResearch, West Grove, PA, USA) diluted 1:10,000. Protein A-sepharose was used to precipitate immune complexes in immunoprecipitation experiments.
Rabbit polyclonal anti pyk2
Manufactured by Cell Signaling Technology
Sourced in United States
Rabbit polyclonal anti-Pyk2 is a lab equipment product used to detect the Pyk2 protein. It is a polyclonal antibody raised in rabbits against the Pyk2 protein.
Automatically generated - may contain errors
Lab products found in correlation
2 protocols using rabbit polyclonal anti pyk2
1
Antibody Validation for Protein Detection
2
Antibody Detection Methods for PYK2 and FAK
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The following antibodies were used in this study: rabbit polyclonal anti-PYK2 (3292; Cell Signaling Technology, Danvers, MA, USA), rabbit polyclonal anti-p-PYK2 (Y402) (3291; Cell Signaling Technology), mouse monoclonal anti-p-PYK2 (Y402) (sc-293142; Santa Cruz Biotechnology, Santa Cruz, CA, USA), rabbit polyclonal anti-FAK (3285; Cell Signaling Technology), rabbit polyclonal anti-p-FAK (Tyr397) (3283; Cell Signaling Technology), and mouse monoclonal anti-glyceraldehyde 3-phosphate dehydrogenase (GAPDH) (G8795; Sigma-Aldrich, St. Louis, MO, USA).
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