Cleaved parp antibody

BA (Meilunbio, Dalian, China; MB5971), Chloroquine (Sigma, St. Louis, MO, USA; C6628), E-64d (Sigma, E8640) and Pepstain A (Sigma, P4265) were dissolved in DMSO (Sigma, D2650). Acridine orange (Sigma, A6014) and 3-MA (Meilunbio, MB5063) were dissolved in PBS. MG132 (Meilunbio, MB5137), Etoposide (Meilunbio, MB1102-S) and CHX (Meilunbio, MB2208) were dissolved in DMSO. LC3 (MBL, Beijing, China; PM036) was bought for immunoblot or immunofluorescence analysis. Antibodies including AKT, phosphorylation of AKT (Ser473; 4060), MTOR, phosphorylation of MTOR (Ser2443; 5535), PARP (116 and 89 kd; 9542S), BAX (5023), phosphorylation of p53 (Ser15; 9286) and GAPDH (Santa Cruz Biotechnology(CA, USA) 5174) were purchased from CST (Beverly, MA, USA). Cleaved-PARP antibody was purchased from Sigma (SAB4500487). p53 (SC-126) and LAMP1 (SC-5570) were purchased from Santa Cruz Biotechnology (CA, USA) for immunoblot or immunofluorescence analyses.

Cypermethrin, cleaved-PARP antibody and N-acetylcysteine (NAC) were purchased from Sigma; γH2AX antibody was purchased from Millipore (Billerica, Bedford, MA, USA); Mouse- or rabbit-monoclonal antibodies specific for phospho-JNK1/2, JNK1/2, phospho-ERK1/2, ERK1/2, p53, p21, cyclin D1, cyclin E, and CDK4 were purchased from Cell Signaling Technology (Boston, MA, USA). 2, 7-dichlorofluorescin diacetate (DCFH-DA) was purchased from Sigma (St. Louis, MO, USA). The Annexin V-fluoresce isothiocyanate (FITC)/propidium iodide (PI) apoptosis detection kit, cell cycle staining kit and glyceraldehyde-3-phosphate dehydrogenase (GAPDH) antibody were obtained from Multisciences Biotechnology (Hangzhou, China).