Td 92034

For sodium appetite feeding studies (Figure S6G,H), male Hsd11b2-Cre mice aged 8–16 weeks were acclimated to a two-diet choice of high sodium diet (1.6% Na⁺ / 4% NaCl; Envigo TD.92034) or standard diet (0.2% Na⁺ / 0.49% NaCl; Envigo TD.96208) for at least 5 days before commencing food intake experiments in the home cage. Because the high sodium diet formulation differed slightly from our in-house standard chow, these experiments used the appropriate control diet matching its formulation offered by the manufacturer. For dark-cycle feeding experiments, the two diets were pre-weighed and male Hsd11b2-Cre mice received a vehicle or CNO injection approximately 10 minutes prior to the onset of dark. Food was again weighed each hour for the next 3 hours and the remaining food weight values were subtracted from the initial pre-weighed food to provide the amount eaten. In water restriction experiments, animals had ad libitum access to high sodium and standard diets and were acclimated to three-hour restricted H₂O access (11:00 – 14:00) for two days prior to the experiment. Next, diets were pre-weighed and animals received vehicle or CNO injections 10 minutes prior to H₂O access. Food intake was measured for the next three hours.