Rabbit anti gfp clone d5

Manufactured by Cell Signaling Technology

Rabbit anti-GFP (clone D5.1) is a primary antibody raised in rabbit against the green fluorescent protein (GFP). It recognizes the GFP protein and can be used for the detection and immunopurification of GFP-tagged proteins.

Automatically generated - may contain errors

Lab products found in correlation

Clone 14 beta catenin, by BD (1 mentions) Histofine simple stain, by Nichirei Biosciences (1 mentions) Bx41 microscope, by Olympus (1 mentions) Mouse anti p44 42 mapk erk1 2 l34f12, by Cell Signaling Technology (1 mentions) Mouse anti gsk3β clone 3d10, by Cell Signaling Technology (1 mentions) Rabbit anti phospho sfk y416 clone 100f9, by Cell Signaling Technology (1 mentions) Rabbit anti ha clone c29f4, by Cell Signaling Technology (1 mentions) Rabbit anti fyn clone epr5500, by Merck Group (1 mentions) Mouse anti bα clone 2g9, by Merck Group (1 mentions) Rabbit anti phospho gsk 3β ser9, by Cell Signaling Technology (1 mentions) Clone c4, by Merck Group (1 mentions) Mouse anti ha clone 16b12, by Fortrea (1 mentions)

Close spelling variants of this product

Anti-GFP (D5.1, Rabbit anti-GFP Anti-GFP

2 protocols using rabbit anti gfp clone d5

Immunostaining of Paraffin-Embedded Sections

Check if the same lab product or an alternative is used in the 5 most similar protocols

Immunostaining of paraffin-embedded sections was performed using primary antibodies against mouse anti-β-catenin (clone 14/Beta-catenin, 610514, BD Biosciences) and rabbit anti-GFP (clone D5.1, 2956, Cell Signaling) diluted 1:100. Slides were then incubated with goat anti-mouse or anti-rabbit Histofine simple stain immuno-enzyme polymers (MAX-PO (M), 414322, and MAX-PO (R), 414341, respectively, Nichirei Bioscience Inc.), and staining was developed using metal-enhanced 3,3′-diaminobenzidine (DAB). Slides were counterstained with hematoxylin and observed under an OLYMPUS BX41 microscope (Olympus Corporation).

Yanai H., Atsumi N., Tanaka T., Nakamura N., Komai Y., Omachi T., Tanaka K., Ishigaki K., Saiga K., Ohsugi H., Tokuyama Y., Imahashi Y., Ohe S., Hisha H., Yoshida N., Kumano K., Kon M, & Ueno H. (2017). Intestinal cancer stem cells marked by Bmi1 or Lgr5 expression contribute to tumor propagation via clonal expansion. Scientific Reports, 7, 41838.

+ Open protocol

+ Expand

Antibody-Based Analysis of Protein Signaling

Cited 1 time

Check if the same lab product or an alternative is used in the 5 most similar protocols

Unless indicated, all chemicals were purchased from Sigma/Merck Millipore. Antibodies used in this study included mouse anti-HA (clone 16B12, Covance); rabbit anti-HA (clone C29F4, Cell Signaling Technology); rabbit anti-GFP (clone D5.1, Cell Signaling Technology); mouse anti-Bα (clone 2G9, Merck Millipore); mouse anti-PP2Ac_α/β (BD Transduction); goat anti-pY307 PP2Ac (Santa Cruz Biotechnology, Inc; denoted SC); rabbit anti-phospho-SFK (Y416) (clone 100F9, Cell Signaling Technology); rabbit anti-phospho-p44/42 MAPK (clone D13.14.4E, Cell Signaling Technology); mouse anti-p44/42 MAPK (ERK1/2) (L34F12, Cell Signaling Technology); rabbit anti-phospho-GSK-3β (Ser 9) (Cell Signaling Technology) and mouse anti-GSK3β (clone 3D10, Cell Signaling Technology); rabbit anti-Src (clone 32G6, Cell Signaling Technology); mouse anti-Src (clone GD11, Merck Millipore); rabbit anti-Fyn (clone EPR5500, Merck Millipore); mouse anti-Fyn (BD Transduction and Cell Signaling Technology); mouse and rabbit anti-p-Tyr (P-Tyr-100 and P-Tyr-1000, Cell Signaling Technology); rabbit anti-Tau (rPeptide), mouse anti-pSer202 Tau (CP13) (59 (link)) (a gift from Peter Davies). Mouse (clone C4, Merck Millipore) or rabbit (Cytoskeleton) anti-actin, and mouse (clone 236-10501, Life Technologies Australia Pty Ltd) or rabbit (Epitomics) anti-α-tubulin antibodies were used for protein normalization.

Sontag J.M., Schuhmacher D., Taleski G., Jordan A., Khan S., Hoffman A., Gomez R.J., Mazalouskas M.D., Hanks S.K., Spiller B.W., Sontag E, & Wadzinski B.E. (2022). A new paradigm for regulation of protein phosphatase 2A function via Src and Fyn kinase–mediated tyrosine phosphorylation. The Journal of Biological Chemistry, 298(8), 102248.

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!