Hexane, heptane, toluene, xylene, chloroform, cycloHexane, diethyl ether, sodium hydroxide and hydroxylamine hydrochloride were purchased from Sigma Aldrich (Missouri, USA). Lipozyme TL IM was obtained from Novo Nordisk (Bagsvaerd, Denmark). American castor oil was purchased from Aria Starr Beauty Co., Sunny California, USA. Dulbecco’s Modified Eagle Medium (DMEM) was purchased from Gibco. Eagle’s Minimum Essential Medium (EMEM), Human Dermal Fibroblasts (HDF, CCL-110), Human Malignant Melanoma (CRL-1619) and Human Glioblastoma Multiforme (CRL-1690) were ordered from ATCC. MTS cell proliferation assays (CellTiter 96 AQueous One Solution Cell Proliferation Assay) were purchased from Promega. A LIVE/DEAD™ Viability/Cytotoxicity Kit for mammalian cells was purchased from Invitrogen. A DCFDA Cellular ROS Detection Assay Kit was ordered from Abcam.
Lipozyme tl im
Manufactured by Novo Nordisk
Sourced in Denmark
Lipozyme TL IM is a lipase enzyme immobilized on a granular, macroporous acrylic resin. It is designed for use in the food and pharmaceutical industries to catalyze various lipid-related reactions.
Automatically generated - may contain errors
Lab products found in correlation
Lipozyme rm im, by Novo Nordisk (3 mentions)
Vinyl laurate, by Merck Group (2 mentions)
Live dead viability cytotoxicity kit for mammalian cells, by Thermo Fisher Scientific (1 mentions)
Dulbecco s modified eagle medium dmem, by Thermo Fisher Scientific (1 mentions)
Dcfda cellular ros detection assay kit, by Abcam (1 mentions)
Hexane, by Merck Group (1 mentions)
Celltiter 96 aqueous one solution cell proliferation assay, by Promega (1 mentions)
Sodium methoxide, by Merck Group (1 mentions)
Boron trifluoride methanol solution, by Merck Group (1 mentions)
Sodium hydroxide (naoh), by Merck Group (1 mentions)
Methanol, by RCI Labscan (1 mentions)
Hexane, by RCI Labscan (1 mentions)
Ethanol, by RCI Labscan (1 mentions)
Deuterated chloroform cdcl3, by Cambridge Isotopes (1 mentions)
Acetone, by RCI Labscan (1 mentions)
Lactose, by Sinopharm (1 mentions)
Maltose, by Sinopharm (1 mentions)
N hexane, by Sinopharm (1 mentions)
Oleyl alcohol, by Merck Group (1 mentions)
Ethanol, by Merck Group (1 mentions)
7 protocols using lipozyme tl im
1
Cytotoxicity and Antioxidant Assays
2
Enzymatic Modification of Cod Liver Oil
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Arctic cod liver oil was purchased from Nordic Naturals (Watsonville, CA, USA). Lipozyme RM IM (Rhizomucor miehei lipase, immobilized on anion exchange resin), Lipozyme TL IM (lipase from Thermomyces lanuginosa) and Novozyme 435 (Lipase acrylic resin from Candida antarctica B) were generous gifts from Novo Nordisk A/S (Bagsvaerd, Denmark). Sodium methoxide, sodium hydroxide, and boron trifluoride-methanol solution (14% in methanol) were obtained from Sigma-Aldrich (Singapore).
Acetone, hexane, ethanol and methanol were acquired from RCI Labscan Limited (Bangkok, Thailand). Deuterated chloroform (CDCl 3 ) containing tetramethylsilane (TMS, v/v 0.03%) was purchased from Cambridge Isotope Laboratories Inc. (Tewksbury, MA, USA).
Acetone, hexane, ethanol and methanol were acquired from RCI Labscan Limited (Bangkok, Thailand). Deuterated chloroform (CDCl 3 ) containing tetramethylsilane (TMS, v/v 0.03%) was purchased from Cambridge Isotope Laboratories Inc. (Tewksbury, MA, USA).
3
Enzymatic Synthesis of Fatty Acid Esters
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Lipozyme TLIM was purchased from Novo Nordisk (Denmark). Molecular sieves (4 Å), 2-methylbutanol, dimethylsulfoxide (DMSO), sucrose, maltose, lactose and n-hexane were from Sinopharm (China). Raffinose pentahydrate was from Alfa Aesar (France). Vinyl laurate and 6-O-monolaurate were from Sigma (Denmark). Vinyl octanoate and vinyl decanoate were purchased from TCI (Japan). Ryoto sucrose ester (L1695) was supplied by Mitsubishi-Kasei Food Corporation (Japan). All the reagents and solvents were of analytical grade. Vinyl fatty acid esters, 2-methyl-2-butanol and DMSO were stored over molecular sieves (4 Å), at least 24 h prior to use. Double-distilled water with a surface tension equal to 69.3 mN/m at 25°C was used in all experiments.
4
Enzymatic Modification of Palm Oil
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Immobilized enzyme, Lipozyme TL IM (commercial lipase from Thermomyces lanuginosus immobilized on silica gel) and Lipozyme RM IM (commercial lipase from Rhizomucor miehei immobilized on microporous anionic exchange resin) were purchased from Novo-Nordisk, Denmark. Refined bleached deodorized (RBD) palm oil was obtained from Golden Jomalina Sime Darby, Malaysia. Fatty acids in Malaysian palm oil are 0.1-0.3％ of lauric acid, 0.9-1.5％ of myristic acid, 39.2-45.2％ of palmitic acid, 3.7-5.1％ of stearic acid, 37.5-44.1％ of oleic acid and 8.7-12.5％ of linoleic acid. Oleyl alcohol, n-hexane and ethanol were purchased from Merck, Germany. All other chemicals were used without further purification and were of analytical grade.
5
Enzymatic Modification of Algal Oil Lipids
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Lipase UM1 was obtained from Guangdong Youmei Institute of Intelligent Bio-manufacturing Co., Ltd. (Foshan, China) . Novozym 435 (Lipase B from Candida antarctica, immobilized on macroporous acrylic resin) , Lipozyme TL IM (Lipase from Thermoanaerobacter ligninum, immobilized on silica gel) , Lipozyme RM IM (lipase from Rhizopus oryzae, immobilized on macroporous anion-exchange resin) were purchased from Novo Nordisk (Bagsvaerd, Denmark) . Standards of triacylglycerol (TAG) , diacylglycerol (DAG) ( 15％ of 1,2-diacylglycerol, 85％ of 1,3-diacylglycerol, w/v) , 1 (3) -monoacylglycerol (MAG) , Eicosapentaenoic acid ( E PA ) ethyl ester, DHA ethyl ester, docosapentaenoic acid (DPA) ethyl ester, and 37 fatty acid methyl esters (C4-C24) for analysis were purchased from Sigma-Aldrich Chemical Co. Ltd. (Shanghai, China) . All other chemicals were of chromatographic and analytical grades. Thin layer chromatographic plate was purchased from Qingdao Haiyang Chemical Co., Ltd. (Qingdao, China) . DHA-rich refined algal oil from Schizochytrium sp. was donated by Carbisol Biotech Co Ltd (Wuhan, China) and stored in sealed aluminium cans at -20℃ before use.
6
Enzymatic Synthesis of Sucrose Esters
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Sucrose and vinyl laurate with >98% purity were purchased from Sigma-Aldrich (Shanghai, China). Sucrose monolaurate (≥97%) was obtained from Sigma-Aldrich (Shanghai, China). Lipozyme TL IM (T. lanuginosus lipase immobilized on silica gel) was a gift from Novo Nordisk (Copenhagen, Denmark). 3 Å and 4 Å Molecular sieves were purchased from Sinopham Chemical Reagent Co., Ltd. (Shanghai, China). Ionic liquid, 3CIM(EO)][NTf2] (1-(2-(2-methoxyethoxy)ethyl)-5,6,7,8-tetrahydroimidazo[1,2-a]pyridin-1-ium bis(trifluoromethylsulfony)imide) (99%, HPLC) was synthesized and purified in our laboratory. All other reagents were of analytical grade. All other chemicals of analytical or HPLC grade were purchased from commercial sources in China. vinyl laurate and other organic solvents were stored over 4 Å molecular sieves, at least 24 h prior to use.
7
Enzymatic Hydrolysis of Safflower Oil
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Edible safflower oil (SO) was purchased from a local market; its composition in fatty acids (%) was determined by gas chromatography (GC) to be 7.5, 3.8, 16.5, and 72.2 of C16:0, C18:0, C18:1, and C18:2, respectively. Two non-immobilized lipases from Candida rugosa type VII (CRL) and from porcine pancreas type II (PPL) as well as sodium methoxide (CH3ONa) were purchased from Sigma Chemical Co. (St. Louis, MO). The hydrolytic activity for CRL was 1,449 unit/mg solid enzyme, whereas that for PPL was 59.45 unit/mg solid enzyme when olive oil is used at pH 7.7. In addition, an immobilized lipase from Thermomyces lanuginosus (Lipozyme TL IM), with 20 unit/mg solid enzyme, was obtained from Novo Nordisk (Copenhagen, Denmark). Both PPL and Lipozyme TL IM are 1,3-regiospecific lipases, whereas CRL is a nonspecific lipase. Polyoxyethylene sorbitan monolaurate (Tween-20), sodium hydroxide (NaOH), Sigma 7-9, Whatman 1PS filter, and organic solvents of high-performance liquid chromatography (HPLC) grade were purchased from Fisher Scientific (Fair Lawn, NJ). 12 N Hydrochloric acid (HCl), 12 N sulfuric acid (H2SO4), and 1 N standard HCl solution were also purchased from Fisher Scientific. Fatty acid methyl ester (FAME), thin-layer chromatography (TLC), and HPLC standards were purchased from Nu-Chek Prep (Elysian, MN).
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