Ab108665

Cellulose papers (CFP42‐457) were purchased from STERLITECH., Inc, USA as the wicking pads. The flexible TPE layer (9984 Diagnostic Microfluidic Surfactant Free Medical Hydrophilic Film), biocompatible adhesive (Medical Tape 1522, Double Sided Transparent Polyethylene), transparent acrylate adhesives (Microfluidic Diagnostic Tape, 9969 Adhesive Transfer Tape), and a waterproof adhesive layer (Tegaderm Transparent Film Dressing) were provided by 3M, Co, USA. The PSA (AR care @8939 as a polyester film with a double‐sided medical pressure‐sensitive adhesive) was purchased from Adhesives Research., Inc, USA. The lateral flow nitrocellulose (Vivid 90 LFNC, VIV92503R, the wicking rate of 70–110 s cm⁻⁴) was provided by PALL., Co, NY, USA, and the microfiber glass (Grade 121,) was supplied by Ahlstrom‐Munksjö., Co, Finland. The Human Cortisol ELISA Kits were purchased from two suppliers: LS‐F10024 purchased from Life Span Biosciences., Co, USA, and ab108665 purchased from Abcam., Ltd, Canada.

The serum cortisol level was estimated using an enzyme‐linked immunosorbent assay (ab108665, Abcam, Cambridge, UK). At the end of the treatment, rats were dissected and blood was collected and processed with the serum for the determination of cortisol level.