The NST 2-hydroxy-N-[2-(1H-indol-3-yl)-ethyl]-benzamide was obtained by “in house” synthesis as previously reported (João Pessoa, PB, Brazil; patent document: BR 200304393-A) in Laboratory of Pharmaceutical Chemistry, UFPI (Teresina, PI, Brazil) [5 (link)]. Carrageenan, acetic acid, Triton X-100, L-methionine, hydroxylamine chloride, riboflavin, thiobarbituric acid (TBA), Griess reagent, sodium dodecylsulfate, O-dianisidine, hexadecyltrimethylammonium bromide (HTAB), sodium phosphate, 5,5′-dithiobis-(2-nitrobenzoic acid) (DTNB), and sodium nitrite were purchased from Sigma-Aldrich (Saint Louis, MO, USA). Indomethacin (Indocid®), sodium phosphate, zinc sulfate, hydrogen peroxide, Tris base, sodium hydroxide, and trichloroacetic acid were purchased from Merck (Darmstadt, Germany). Turk's solution was purchased from Newprov (Pinhais, PR, Brazil).
Hydroxylamine chloride
Manufactured by Merck Group
Sourced in United States, Spain
Hydroxylamine chloride is a chemical compound used in various laboratory applications. It is a solid, crystalline substance with the chemical formula NH2OH·HCl. Hydroxylamine chloride serves as a reducing agent and can be used in various analytical and synthetic procedures.
Automatically generated - may contain errors
Lab products found in correlation
Trichloroacetic acid, by Merck Group (3 mentions)
Sodium hydroxide (naoh), by Merck Group (3 mentions)
Hydrogen peroxide, by Merck Group (3 mentions)
Thiobarbituric acid, by Merck Group (3 mentions)
Phenyl β glucoside, by Merck Group (3 mentions)
Sucrose, by Merck Group (3 mentions)
Fructose, by Merck Group (3 mentions)
Trifluoroacetic acid tfa, by Merck Group (3 mentions)
Myo inositol, by Merck Group (3 mentions)
Sodium dodecyl sulfate (sds), by Merck Group (2 mentions)
Raffinose, by Merck Group (2 mentions)
Hexamethyldisilazane hmds, by Merck Group (2 mentions)
Pinitol, by Merck Group (2 mentions)
Nitroblue tetrazolium chloride, by Merck Group (2 mentions)
Potassium dihydrogen phosphate, by Merck Group (2 mentions)
Sodium dihydrogen phosphate, by Merck Group (2 mentions)
Reduced glutathione, by Merck Group (2 mentions)
Sodium carbonate, by Merck Group (2 mentions)
Ethylenediaminetetraacetic acid, by Merck Group (2 mentions)
Ketamine, by Merck Group (2 mentions)
8 protocols using hydroxylamine chloride
1
Synthesis and Evaluation of NST Compound
2
Analytical Standards for Metabolomic Studies
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Analytical standards of arabinose, arabitol, L-ascorbic acid, citric acid, fructose, glucose, galactose, (-)-calcium hydroxycitrate tribasic [(-)-HCA], (-)-hydroxycitric acid lactone [(-)-HCAL], muco-inositol, myo-inositol, maltose, maltotriose, maltotetraose, mannitol, pinitol, phenyl-β-glucoside (internal standard), raffinose, sucrose, and verbascose were obtained from Sigma Aldrich (St. Louis, MO, USA). Derivatization reagents including hydroxylamine chloride, anhydrous pyridine, hexamethyldisilazane (HMDS), and trifluoroacetic acid (TFA) were also acquired from Sigma Aldrich (St. Louis, MO, USA).
3
Synthesis and Analysis of M3OMG and P3OMG
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Methyl gallate, borax, dimethyl sulphate, dipropyl sulphate, sodium hydroxide, sulphuric acid, chloroform, sodium chloride and sodium sulphate were purchased from Sigma-Aldrich (St Louis, MO, USA) for the synthesis of M3OMG and P3OMG. Bovine serum albumin and a kit for protein measurement were purchased from ZiestChem Company (Tehran, Iran). 5,5-dithiobis(2-nitrobenzoic acid), ethylenediaminetetraacetic acid, nitro blue tetrazolium chloride, potassium dihydrogen phosphate, reduced glutathione, sodium dihydrogen phosphate, trichloroacetic acid, thiobarbituric acid, hydrogen peroxide, sodium carbonate, hydroxylamine chloride, ketamine, lidocaine, xylazine were purchased from Sigma-Aldrich Chemical Company, (St. Louis, MO, USA). Other chemical reagents and solvents were of analytical grade or pure and were purchased from Merck Chemical Company (Darmstadt, Germany).
4
Analytical Standards for Carbohydrates and Phenols
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Analytical standards of pinitol, myo-inositol, galactinol, glucose, fructose, sucrose, raffinose, stachyose, and phenyl-β-D-glucopyranoside were purchased from Sigma Chemical Co. (St. Louis, MO, USA). Ethanol and mEthanol were acquired from Scharlab (Barcelona, Spain) and hydroxylamine chloride, hexamethyldisilazane and trifluoroacetic acid were purchased from Sigma Chemical Co. (St. Louis, MO, USA)
5
Comparative Analysis of GABA Green and Oolong Tea
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GABA green tea and GABA oolong tea were purchased from an Italian specialist tea shop (La Teiera Eclettica, Milan, Italy). Gallic acid, epigallocatechin-gallate, epicatechin-gallate, theanine, glutamic acid and glutamine were obtained from PhytolabGmbH and Co., KG (Vestenbergsgreuth, Germany). Bovine serum albumin and a kit for protein determination were purchased from ZiestChem Company (Tehran, Iran). In addition, 5,5-dithiobis(2-nitrobenzoic acid), ethylenediaminetetraacetic acid, nitro blue tetrazolium chloride, potassium dihydrogen phosphate, reduced glutathione, sodium dihydrogen phosphate, trichloroacetic acid, thiobarbituric acid, hydrogen peroxide, sodium carbonate, hydroxylamine chloride, ketamine, lidocaine, xylazine, FolinCiocalteau’s reagent, LC/MS-grade methanol and acetonitrile, formic acid solution 1 M, caffeine, epicatechin, catechin, sodium acetate, trimethylamine, tetrahydrofuran, ortho-phthalaldehyde, fluorenylmethylchloroformate were purchased from Sigma-Aldrich Chemical Company (St. Louis, MO, USA). Other chemical reagents and solvents were purchased from Merck Chemical Company, Darmstadt, Germany.
6
Comprehensive Carbohydrate Analysis Protocol
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HMP was acquired from Frutarom SA (Lima-Perú). Ascorbic acid (Aa), ammonium acetate, β-phenyl glucoside, hydroxylamine chloride, potassium iodide and trifluoroacetic acid were purchase from Sigma-Aldrich (St. Louis, MO, USA). Monosaccharide standards arabinose (Ara), fucose (Fu), fructose (Fs), galactose (Gal), galacturonic acid (GalA), glucose (Glc), mannose (Man), raffinose (Raf), rhamnose (Rha), sucrose (Suc) and xylose (Xyl), and sodium dodecyl sulfate (SDS) were acquired from Sigma-Aldrich (St. Louis, MO, USA).
7
Analytical Standards for Artichoke Biomass
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Analytical standards of glucose, fructose, sucrose, myo-inositol, scyllo-inositol, chiroinositol, quinic acid, caffeic acid, chlorogenic acid and phenyl-β-glucoside (internal standard) were obtained from Sigma Aldrich (Madrid, Spain). Derivatization reagents including hydroxylamine chloride, anhydrous piridine, hexamethyldisilazane (HMDS), trifluoroacetic acid (TFA) and N,O-bis(trimethylsilil)trifluoroacetamide (BSTFA) with 1% trimethylchlorosilane (TMCS) were also acquired from Sigma Aldrich (Madrid, Spain).
Fresh artichokes (var. Blanca de Tudela) from 4 different brands were purchased at local markets (Madrid, Spain) and then divided into external bracts (B1-B4) and receptacles (R1-R3). Fresh leaves (L1, L2, L4-L6) and stalks (S1-S2) were collected from local plantations. An artichoke sapling (young plant) was also bought on internet and leaves were collected (L3). The samples were freeze-dried and then grounded to fine particles in a domestic mill (Moulinex, Spain) and sieved through a 500 µm mesh.
Pools of leaves (PL), external bracts (PB), stalks (PS) and receptacles (PR) were also prepared by mixing equal amounts of each individual sample. All samples were stored in dry and hermetically closed recipients protected from light at ambient temperature until extraction.
Fresh artichokes (var. Blanca de Tudela) from 4 different brands were purchased at local markets (Madrid, Spain) and then divided into external bracts (B1-B4) and receptacles (R1-R3). Fresh leaves (L1, L2, L4-L6) and stalks (S1-S2) were collected from local plantations. An artichoke sapling (young plant) was also bought on internet and leaves were collected (L3). The samples were freeze-dried and then grounded to fine particles in a domestic mill (Moulinex, Spain) and sieved through a 500 µm mesh.
Pools of leaves (PL), external bracts (PB), stalks (PS) and receptacles (PR) were also prepared by mixing equal amounts of each individual sample. All samples were stored in dry and hermetically closed recipients protected from light at ambient temperature until extraction.
8
Enzymatic Assay for β-Galactosidase
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Commercial β-galactosidase (β-gal) HA-Lactase from K. lactis was provided by CHR Hansen (Bogotá, Colombia), commercial glucose oxidase (Gox) Gluzyme from Aspergillus oryzae was a generous gift from Novozymes (Bagsvaerd, Denmark). Glucose (Glc), lactose (Lac), galactose (Gal), phenyl-β-D-glucoside, hydroxylamine chloride, pyridine, hexamethyldisilazane (HDMS), trifluoroacetic acid (TFA), bovine serum albumin (BSA), sodium dihydrogen phosphate monohydrate, disodium hydrogen phosphate dihydrate, and sodium hydroxide were purchased from Sigma-Aldrich (St. Louis, MO).
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