Ht 2 toxin

There were 17 analytical standards of mycotoxin used in the experiments: 1) Fusarium toxins: nivalenol, deoxynivalenol, T-2 toxin, HT-2 toxin and zearalenone were obtained from Biopure (Romer Labs, Tulln, Austria) and the other Fusarium toxins consisting of 15-acetyldeoxynivalenol, 3-acetyldeoxynivalenol, fusarenon X, neosolaniol, fumonisin B1 and B2 were obtained from Trilogy Lab (Washington, MO., USA) and diacetoxyscirpenol from Cayman Chemical (Cayman Chemical Ltd., Michigan, MI, USA); 2) Aspergillus and Penicillium toxins: aflatoxins (B1, B2, G1, G2) were obtained from Sigma Aldrich (St. Louis, MO, USA) and ochratoxin A from Biopure (Romer Labs, Tulln, Austria).

DELFIA series buffers, streptavidin and rabbit anti-mouse-coated microtiter plates were purchased from Kaivogen Diagnostics (Turku, Finland). All measurements were done with a Victor 1420–fluorometer from Perkin-Elmer (Turku, Finland). The magnetic nanoparticles and magnetic bead concentrator were purchased from Dynal (Norway). The mycotoxins nivalenol (NIV), Deoxynivalenol (DON), 3-Acetyldeoxynivalenol (3-AcDON), 15-Acetyldeoxynivalenol (15-AcDON), T-2 toxin and HT-2 toxin were purchased from Biopure Guntramsdorf, Austria). Hyperphages were obtained from Thermo Fisher Scientific (Waltham, MA, USA). The E. coli cell lines used for the sorting and expression of the antibody libraries were purchased from Stratagene (La Jolla, CA, USA): BL21 (F-, dcm, ompT, hsdS[rB− mB−], gal [malB+], K-12[λS]) and XL1-Blue (recA1, endA1, gyrA96, thi-1, hsdR17, relA1, lac [F`, TetR]). All microbiological reagents were prepared as described in Sambrook et al. [16 ]. The single-chain alkaline phosphatase (scFv-ALP) fusion proteins were purified with HisPur Ni-NTA spin columns Thermo Fisher Scientific (Waltham, MA, USA). The ELISA substrate para-nitrophenylphosphate (pNPP) was obtained from Sigma-Aldrich (St. Louis, MO, USA).

Ochratoxin A, deoxynivalenol, nivalenol, T-2 toxin, HT-2 toxin, diacetoxyscirpenol (DAS), zearalenone (ZEN), ¹³C-DON, ¹³C-T-2, ¹³C-HT-2 toxins, and ¹³C-ZEN were purchased from Biopure (Tulln, Austria). Ammonium acetate, Celite^® 545, acetic acid, acetonitrile (ACN; gradient grade), and methanol (MeOH; MS grade) were supplied by Merck-Millipore (Darmstadt, Germany). The Simplicity 185 (Millipore, Bedford, MA, USA) water purification system was used for the deionized water production.