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Las af 1.5.1 acquisition software

Manufactured by Leica
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LAS AF 1.5.1 is an acquisition software for Leica microscopy systems. The software provides functionality for image capture and basic image processing.

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Lab products found in correlation

Dfc350fx, by Leica (1 mentions) Plan apochromat 100, by Leica (1 mentions)

Close spelling variants of this product

LAS AF acquisition software LAS AF software LAS AF 1.8.2 software LAS AF LAS software

2 protocols using las af 1.5.1 acquisition software

1

Chromosomal Tagging of Exocyst Complex

Cited 1 time
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Chromosomal tagging of SEC5, SEC8, EXO70 and EXO84 by Green Fluorescence Protein (GFP) was performed as previously described [38 (link)]. Cells were grown to log phase at 25 °C. The cells were then fixed for microscopy as described previously [39 (link)]. F-actin was stained with Alexa Fluor 488-conjugated phalloidin (Molecular Probes Corp.). Fluorescence microscopy was conducted with microscope CTR6000 (Leica) equipped with a Plan-Apochromat 100x, 1.40 NA oil immersion objective lens. Images were taken using LAS AF 1.5.1 acquisition software (Leica).
Liu Y., Zhang T., Sun D, & Luo G. (2019). The Cdc42 effectors Gic1 and Gic2 regulate polarized post-Golgi secretion. Cell & Bioscience, 9, 33.
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2

Fluorescence Microscopy of Log-Phase Cells

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Fluorescence microscopy was performed with a Leica CTR6000 fluorescence microscope equipped with a Plan-Apochromat 100 × , 1.40 NA oil immersion objective lens. Images were taken using LAS AF 1.5.1 acquisition software (Leica). Cells were grown to early log phase (0.6 OD600) in synthetic complete media and fixed by methanol/acetone before microscopy. Images were captured with a digital camera (DFC350FX; Leica) at room temperature.
Yue P., Zhang Y., Mei K., Wang S., Lesigang J., Zhu Y., Dong G, & Guo W. (2017). Sec3 promotes the initial binary t-SNARE complex assembly and membrane fusion. Nature Communications, 8, 14236.
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