6520 q tof lc ms mass spectrometer

IR spectra were recorded on an Avater-360 FT-IR spectrophotometer with KBr pellets. UV spectra were run on a Carry-50 UV-VIS spectrophotometer.
HRESIMS were measured by an Agilent 6520 Q-TOF LC-MS mass spectrometer. 1D-and 2D-NMR spectra were recorded on a Bruker AV-400 spectrometer (400 MHz for 1 H and 100MHz for 13 C, respectively).
Semi-preparative HPLC was performed on a Hitachi Spectra Series HPLC system equipped with an L-2130 pump and a UV L-2400 detector in a YMC-ODS column (10 mm × 250 mm, 5 μm); flow rate at 1.5 mL/min; wavelength detection at 254 nm. Fractions were monitored by TLC, and spots were visualized by heating silica gel plates after spraying with 10% H 2 SO 4 in EtOH. GC analysis was carried out on an Agilent 7820A GC system using an HP-5 (30 m × 0.32 mm × 0.25 μm) column; detection FID; carrier gas N 2 ; injection temperature 250℃, detection temperature 250℃, column temperature 180℃. Preparative HPLC methanol and chemicals of analytical grade, such as methanol, dichloromethane, and ethyl acetate, were purchased from Sinopharm Chemical Reagent Co., Ltd. The cell line, RAW264.7 (mouse macrophage), was available and maintained in our laboratory.