HSCs were cultured in media composed of F12 media, 1% ITSX, 1%, 10 mM HEPES, 1% P/S/G, 100 ng/ml mouse TPO, 10 ng/ml mouse SCF and 0.1% of one the following chemicals (all from Sigma): hydroxypropyl cellulose (HPC; 435007), low-viscosity carboxymethylcellulose sodium salt (CMC-LV; C5678), medium viscosity carboxymethylcellulose sodium salt (CMC-MV; 21902), alpha-cyclodextrin (alpha-CD; C4642), beta-cyclodextrin (beta-CD; C4767), gamma-cyclodextrin (gamma-CD; C4930), 2-hydroxypropyl-betal—cylodextrin (HBC; H107), 2-hydroxypropyl-gamma-cycldextrin (HGC; H125), methyl-beta-cylodextrin (MBC; C4555), poloxamer 188 (polox188; P5556), or polyvinyl alcohol (PVA; P8136, 363081, or 363146), at 37°C with 5% CO2. For long-term cultures in PVA-based cultures, complete media changes were made every 2–3 days after the first 5–6 days, as described above for albumin-based cultures. Long-term cultures were split 1:3 at ~90% confluency. Where indicated, lipopolysaccharide (LPS; Sigma L2762) or IL-6 (Peprotech) were added to cultures.
Alpha cyclodextrin
Manufactured by Merck Group
Sourced in United States
Alpha-cyclodextrin is a cyclic oligosaccharide composed of six glucose units. It is a water-soluble, crystalline compound used in various laboratory applications. Alpha-cyclodextrin serves as a complexing agent, capable of forming inclusion complexes with a wide range of molecules.
Automatically generated - may contain errors
Lab products found in correlation
Hydroxypropyl cellulose, by Merck Group (2 mentions)
Mouse tpo, by Merck Group (2 mentions)
Mouse scf, by Merck Group (2 mentions)
Interleukin 6 (il 6), by Thermo Fisher Scientific (2 mentions)
Ammonium dihydrogen phosphate, by Merck Group (1 mentions)
Imidazole, by Merck Group (1 mentions)
Ortho phosphoric acid, by Scharlab (1 mentions)
N n carbonyldiimidazole cdi, by Merck Group (1 mentions)
Analytical grade acetonitrile, by Honeywell (1 mentions)
Hydrochloric acid (hcl), by Scharlab (1 mentions)
4 protocols using alpha cyclodextrin
1
Expansion of Hematopoietic Stem Cells
2
Alpha-Cyclodextrin Capture Solution
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
The capture solution was 100 mg mL−1 alpha-cyclodextrin (Sigma, Aldrich, MI, USA, C4642) in pure Milli-Q water, unless otherwise indicated. The solution was freshly prepared each day.
3
Expansion of Hematopoietic Stem Cells
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
HSCs were cultured in media composed of F12 media, 1% ITSX, 1%, 10 mM HEPES, 1% P/S/G, 100 ng/ml mouse TPO, 10 ng/ml mouse SCF and 0.1% of one the following chemicals (all from Sigma): hydroxypropyl cellulose (HPC; 435007), low-viscosity carboxymethylcellulose sodium salt (CMC-LV; C5678), medium viscosity carboxymethylcellulose sodium salt (CMC-MV; 21902), alpha-cyclodextrin (alpha-CD; C4642), beta-cyclodextrin (beta-CD; C4767), gamma-cyclodextrin (gamma-CD; C4930), 2-hydroxypropyl-betal—cylodextrin (HBC; H107), 2-hydroxypropyl-gamma-cycldextrin (HGC; H125), methyl-beta-cylodextrin (MBC; C4555), poloxamer 188 (polox188; P5556), or polyvinyl alcohol (PVA; P8136, 363081, or 363146), at 37°C with 5% CO2. For long-term cultures in PVA-based cultures, complete media changes were made every 2–3 days after the first 5–6 days, as described above for albumin-based cultures. Long-term cultures were split 1:3 at ~90% confluency. Where indicated, lipopolysaccharide (LPS; Sigma L2762) or IL-6 (Peprotech) were added to cultures.
4
Synthesis of alpha-cyclodextrin conjugates
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Alpha-cyclodextrin, N,N′-carbonyldiimidazole (CDI), imidazole, and ammonium dihydrogen phosphate were purchased from Sigma Aldrich (St. Louis, MO, USA). Milli Q water was generated using an Ultramatic Wasserlab purification system (Pamplona, Spain). For pH adjustment, 37% hydrochloric acid and 85% ortho-phosphoric acid (Scharlab, Madrid, Spain) were used. Analytical grade acetonitrile was purchased from Honeywell (Charlotte, NC, USA). Bentonites and zeolites were kindly provided by Nurel S.A. (Zaragoza, Spain).
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!