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Mouse pcsk9 elisa kit

Manufactured by R&D Systems
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The Mouse PCSK9 ELISA kit is a quantitative sandwich enzyme-linked immunosorbent assay (ELISA) designed for the measurement of mouse PCSK9 (Proprotein Convertase Subtilisin/Kexin Type 9) levels in biological samples. This kit utilizes a specific antibody coated on a microplate to capture PCSK9 from the samples, and a second antibody conjugated with an enzyme for detection.

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Lab products found in correlation

Protein g coated magnetic beads, by Thermo Fisher Scientific (2 mentions)

Close spelling variants of this product

PCSK9 ELISA kit Mouse ELISA kits PCSK9 ELISAs

6 protocols using mouse pcsk9 elisa kit

1

Quantifying Serum PCSK9 Levels

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Serum total PCSK9 level was tested by a mouse PCSK9 ELISA kit (R&D Systems, Minneapolis, MN, USA) comparing experimental sera samples to an internal standard curve according to manufacturer's protocol.
Wu D., Zhou Y., Pan Y., Li C., Wang Y., Chen F., Chen X., Yang S., Zhou Z., Liao Y, & Qiu Z. (2019). Vaccine Against PCSK9 Improved Renal Fibrosis by Regulating Fatty Acid β‐Oxidation. Journal of the American Heart Association: Cardiovascular and Cerebrovascular Disease, 9(1), e014358.
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2

Serum Lipid and PCSK9 Profiling

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Serum total cholesterol, HDL-cholesterol and triglycerides were measured from serum samples collected at the time of termination of the experiments using StanBio kits according to the manufacturers’ guidelines. Mouse serum PCSK9 was measured from the mouse serum samples using R&D Biosystems, mouse PCSK9 ELISA kit according to the manufacturers’ guidelines.
Shende V.R., Singh A.B, & Liu J. (2015). A Novel Peroxisome Proliferator Response Element Modulates Hepatic Low Density Lipoprotein Receptor Gene Transcription in Response to PPARδ Activation. The Biochemical journal, 472(3), 275-286.
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3

Quantifying Plasma PCSK9 Levels

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Plasma PCSK9 level was tested by a mouse PCSK9 ELISA kit (R&D Systems) by comparing experimental sera samples to an internal standard curve according to manufacturer’s protocol. PCSK9 level was quantified both before and after removal of immunoglobulin using Protein G-coated magnetic beads (Life Technologies). Plasma samples were incubated for 10 min with magnetic Protein G beads at room temperature. The Protein G beads were isolated using a magnet, and the immunoglobin-free supernatant was then used directly in PCSK9 ELISA kit.
Pan Y., Zhou Y., Wu H., Chen X., Hu X., Zhang H., Zhou Z., Qiu Z, & Liao Y. (2017). A Therapeutic Peptide Vaccine Against PCSK9. Scientific Reports, 7, 12534.
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4

In Vivo CRISPR-Cas12a Delivery and Analysis

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C57BL/6 male mice (5 to 6 weeks) were intravenously injected with the assemblies. The mice were divided into three groups (n = 5): PBS, Cas12a/crRNA-control/NC-control/PEI/Gal-PEI-DM, and Cas12a/crRNA-exon3/NC-exon3/PEI/Gal-PEI-DM. Each mouse was injected with assemblies containing 50 μg of Cas12a (~2.5 mg/kg mouse) every other day (days 1, 3, and 5) for three times. The mice were fed under normal conditions for another week until the extraction of tissue and blood samples (day 12). After overnight fasting (day 13), the mice were euthanized for liver and peripheral blood collection. From the serum samples, PCSK9 levels were determined using the Mouse PCSK9 ELISA Kit (R&D Systems). Serum cholesterol, ALT, and albumin levels were tested at the UNC Histopathology and Laboratory Medicine. Genomic DNA of the collected livers was extracted for T7EI assay and deep sequencing. Other major organs of the mice were also harvested for H&E staining to assess potential pathological abnormalities.
Sun W., Wang J., Hu Q., Zhou X., Khademhosseini A, & Gu Z. (2020). CRISPR-Cas12a delivery by DNA-mediated bioresponsive editing for cholesterol regulation. Science Advances, 6(21), eaba2983.
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5

Quantifying Secreted PCSK9 in Mice

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Secreted PCSK9 in sera of C57BL/6J mice were measured using mouse PCSK9 ELISA kit obtained from R&D System according to the instruction.
Dong B., Singh A.B., Azhar S., Seidah N.G, & Liu J. (2015). High-fructose feeding promotes accelerated degradation of hepatic LDL receptor and hypercholesterolemia in hamsters via elevated circulating PCSK9 levels. Atherosclerosis, 239(2), 364-374.
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6

PCSK9 Quantification in Plasma

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Plasma PCSK9 levels were quantitated by a mouse PCSK9 ELISA kit (R&D Systems) by comparing experimental sera samples diluted 200-fold to an internal standard curve. PCSK9 was quantified in this way both before and after removal of immunoglobulin using Protein G-coated magnetic beads (Life Technologies). Briefly, plasma samples diluted 1:200 were split into equivalent volumes, then either a) incubated for 10 minutes with magnetic Protein G beads or b) set aside at room temperature. The Protein G beads were isolated using a magnet, and the Ig-cleared supernatant was then used directly in the PCSK9 ELISA kit, alongside the untreated plasma sample.
Crossey E., Amar M.J., Sampson M., Peabody J., Schiller J.T., Chackerian B, & Remaley A.T. (2015). A Cholesterol-Lowering VLP Vaccine that Targets PCSK9. Vaccine, 33(43), 5747-5755.
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