Spd 10a uv vis detector

Optical rotations were recorded using a JASCO P-2000 polarimeter with a 1 cm cell. UV spectra were obtained with a Beckman Coulter DU800 spectrophotometer with a path length of 1 cm. IR spectra were recorded on a Perkin-Elmer 1600 FT-IR spectrometer. The 1D and 2D NMR spectroscopic data were obtained on a JEOL 500 NMR spectrometer. X-ray data for 1a were obtained using a Bruker Smart APEX II CCD diffractometer equipped with Cu K_α radiation (λ = 1.54178 Å). The chemical shift values are reported in ppm units and coupling constants are reported in Hz. NMR chemical shifts were referenced to the residual solvent peaks (δ_H 7.16 and δ_C 128.0 for benzend-d₆). High-resolution ESI-TOF mass spectral data were measured on an Agilent 6530 Accurate-Mass Q-TOF LCMS spectrometer coupled to an Agilent 1280 LC system with a Phenomenex Luna C18 column (4.6 × 100 mm, 5 µm, flow rate 0.7 mL/min). Preparative HPLC separations were performed using a Shimadzu SCL-10A chromatograph with a Shimadzu SPD-10A UV/Vis detector and a reversed-phase C18 column (Phenomenex Luna, 10.0 × 250 mm, 10 µm) at a flow rate of 3.0 mL/min.