After the 1 h incubation of CC cells with blocking buffer (5% normal goat serum, 3% bovine serum albumin and 0.1% Triton-X 100 in PBS), the primary antibodies against β-catenin (Abcam, Cambridge, U.K.) were added and incubated with the CC cells overnight. Following rinsing for three times with PBST, cells were then incubated with secondary antibodies for 1 h under room temperature. DAPI (Burlingame, CA) was then used to stain the cell nuclei. Pictures were taken applying the Nikon Ti inverted fluorescence microscope.
Primary antibodies against β catenin
Manufactured by Abcam
Sourced in United States
Primary antibodies against β-catenin. These antibodies recognize and bind to the β-catenin protein, which is a key component of the Wnt signaling pathway and plays a role in cell-cell adhesion.
Automatically generated - may contain errors
Lab products found in correlation
2 protocols using primary antibodies against β catenin
1
Immunofluorescence Staining of β-Catenin
2
Immunofluorescence Analysis of β-catenin
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Transiently transfected MA-891 cells were cultured on cover slips. The cells were washed with 1× phosphate buffered saline (PBS) and fixed with 4% paraformaldehyde for 1 h at room temperature. After being washed with PBS, the cover slips were incubated with 1× PBS containing 5% bovine serum albumin and 1% Triton X-100 for 15 min at room temperature and then incubated with primary antibodies against β-catenin (Abcam, USA, dilution: 1:600) at room temperature for 2 h. They were then washed with 1× PBS for 30 min to remove nonspecific binding, followed by reaction with the secondary antibody, and then a goat anti-mouse immunoglobulin G (IgG)-Texas Red (Invitrogen, USA) was added to the slides. The slides were incubated for 1 h at the room temperature. The nuclei were visualized with DAPI staining. Cell morphology was visualized and photographed with fluorescence microscopy.
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