Cremophor EL, DOCA, dimethyl sulfoxide (DMSO), N,N-dimethylformamide (DMF) and Tween-80; pentobarbitone sodium (Biowet, Puławy, Poland) and sodium chloride (NaCl) (Chempur, Piekary Śląskie, Poland).
Unless otherwise stated, all chemicals were purchased from Sigma-Aldrich (Munich, Germany). U46619, CBD, GW9662, L161982, O-1918, TRAM-34, AM251, and AM630 were bought from Tocris Bioscience (Bristol, UK). Cay10441 was purchased from Cayman Chemical Co. (Ann Arbor, Michigan, USA). Stock solutions of CBD, U46619, GW9662, AM251, O-1918, capsazepine, nimesulide, Cay10441 and RN1734 were made to 10 mmol/l in ethanol; AM630, L161982, UCL1684 and TRAM-34 were made to 10 mmol/l in dimethylsulfoxide. Their final concentrations were prepared by dilutions with deionized water, which adjusted the final concentrations of ethanol or DMSO 0.1% v/v or less with the exception of RN1734 (ethanol final concentration 0.2% v/v). Stock solutions of l -NAME (300 mmol/l), phenylephrine and acetylcholine (all 10 mmol/l) were made in distilled water. Indomethacin was dissolved in 0.5 mol/l NaHCO3 and DETCA directly into Krebs.
Unless otherwise stated, all chemicals were purchased from Sigma-Aldrich (Munich, Germany). U46619, CBD, GW9662, L161982, O-1918, TRAM-34, AM251, and AM630 were bought from Tocris Bioscience (Bristol, UK). Cay10441 was purchased from Cayman Chemical Co. (Ann Arbor, Michigan, USA). Stock solutions of CBD, U46619, GW9662, AM251, O-1918, capsazepine, nimesulide, Cay10441 and RN1734 were made to 10 mmol/l in ethanol; AM630, L161982, UCL1684 and TRAM-34 were made to 10 mmol/l in dimethylsulfoxide. Their final concentrations were prepared by dilutions with deionized water, which adjusted the final concentrations of ethanol or DMSO 0.1% v/v or less with the exception of RN1734 (ethanol final concentration 0.2% v/v). Stock solutions of