The transwell system (24-well insert; pore size 8 mm; Corning Costar) was used to measure the invasive ability of the HEY cell line. The inserts were coated with 50 µL Matrigel at 1:8 dilution (BD Biosciences Pharmingen) overnight, and 3×104 cells suspended in 0.2 mL of fresh medium without FBS were added to the upper well of the chamber. Then, 600 µL of complete medium with 10% FBS was added to the lower well. After incubating for 16 h, the cells on the upper surface of the membrane were swiped off with cotton swabs, and the cells adhering to the lower surface of the inserts were fixed and stained with haematoxylin. The number of stained cells in five representative fields were randomly counted for each insert using an Olympus light microscope at 100× magnification.
Transwell system 24 well insert pore size 8 mm
Manufactured by Corning
The Transwell system (24-well insert; pore size 8 mm) is a cell culture insert designed for various in vitro applications. The system features a permeable membrane with a pore size of 8 micrometers, allowing for the study of cell migration, transport, and interaction between different cell types or cell-matrix interactions. The 24-well format provides a convenient and standardized platform for multiple experiments.
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Lab products found in correlation
3 protocols using transwell system 24 well insert pore size 8 mm
1
Transwell Invasion Assay for HEY Cells
2
Transwell Invasion Assay for HEY Cells
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
The Transwell system (24-well insert; pore size 8 mm; Corning Costar) was used to measure the invasiveness of the HEY cell lines. The inserts were coated with 50 µl of Matrigel (BD Biosciences Pharmingen), and 3×10 4 cells suspended in 0.2 ml of fresh medium without FBS were added to the upper well of the chamber. Then, 600 µl of complete medium with 10% FBS was added to the lower well. After incubating for 16 h, the cells on the upper surface of the membrane were swiped off with cotton swabs, and the cells adhering to the lower surface of the inserts were xed and stained with haematoxylin. The number of stained cells in ve representative elds were randomly counted for each insert using an Olympus light microscope at 100× magni cation.
3
Transwell Invasion Assay for HEY Cells
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
The Transwell system (24-well insert; pore size 8 mm; Corning Costar) was used to measure the invasiveness of the HEY cell lines. The inserts were coated with 50 µl of Matrigel (BD Biosciences Pharmingen), and 3×10 4 cells suspended in 0.2 ml of fresh medium without FBS were added to the upper well of the chamber. Then, 600 µl of complete medium with 10% FBS was added to the lower well. After incubating for 16 h, the cells on the upper surface of the membrane were swiped off with cotton swabs, and the cells adhering to the lower surface of the inserts were xed and stained with haematoxylin. The number of stained cells in ve representative elds were randomly counted for each insert using an Olympus light microscope at 100× magni cation.
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