Rabbit anti lc3b l7543

Manufactured by Merck Group

Sourced in France

Rabbit anti-LC3B (L7543) is a primary antibody that recognizes the microtubule-associated protein 1 light chain 3B (LC3B). LC3B is a widely used marker for autophagy, a cellular process involved in the degradation and recycling of cellular components.

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Lab products found in correlation

Rabbit anti parp 9542, by Cell Signaling Technology (1 mentions) Mouse anti lamp2 sc 18822, by Santa Cruz Biotechnology (1 mentions) Mouse anti lamp1 h4a3, by Santa Cruz Biotechnology (1 mentions) Goat anti β actin sc 1616, by Santa Cruz Biotechnology (1 mentions) Non fat dry milk, by Bio-Rad (1 mentions) Pvdf membrane, by Bio-Rad (1 mentions) Clarity western ecl substrate, by Bio-Rad (1 mentions) Protease and phosphatase inhibitor cocktail, by Roche (1 mentions) Rabbit anti phospho mtor ser2448, by Cell Signaling Technology (1 mentions) Rabbit anti ulk1, by Cell Signaling Technology (1 mentions) Rabbit anti 4e bp1, by Cell Signaling Technology (1 mentions) Rabbit anti p70 s6 kinase, by Cell Signaling Technology (1 mentions) Rabbit anti phospho p70 s6 kinase, by Cell Signaling Technology (1 mentions) Rabbit anti p62, by Merck Group (1 mentions) Rabbit anti mtor, by Merck Group (1 mentions) Mouse anti ve cadherin sc 9989, by Santa Cruz Biotechnology (1 mentions) Mouse anti β actin, by Merck Group (1 mentions) Rabbit anti gapdh, by Merck Group (1 mentions)

Spelling variants of this product

L7543 (102 citations) Anti-LC3B (74 citations) Rabbit anti-LC3B (33 citations) LC3B (L7543) (12 citations) Anti-LC3B (L7543) (11 citations) Rabbit anti- (2 citations)

3 protocols using rabbit anti lc3b l7543

Protein Detection and Autophagy Assays

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Rabbit anti-LC3B (L7543) and mouse anti-betaActin (A5441) were from Sigma-Aldrich (Saint-Quentin-Fallavier, France). Guinea pig anti-p62 (GP62-C) was from PROGEN Biotechnik GmbH (Heidelberg, Germany). Rabbit anti-mTOR (2972S), rabbit anti-p-mTOR (2971S), rabbit anti-p-Akt (4058S), rabbit anti-Akt (9272S) and rabbit anti-PARP (9542) were from Cell Signaling (Danvers, MA, USA). Rabbit-anti-p-PERK (sc-32577), rabbit anti-GRP78 (H-129), mouse anti-Lamp1 (H4A3) and mouse-anti-Lamp2 (sc-18822) were from Santa Cruz Biotechnology (Heidelberg, Germany).
ML-9 (105637) was from Enzo Life Sciences (Villeurbanne, France). Acridine orange (A-3568) was from Life Technologies (Grand Island, NY, USA). Bafilomycin A1 (1334) was from Tocris (Bristol, UK). Chloroquine (C6628), Wortmannin (W1628) and trypan blue solution (T8154) were from Sigma-Aldrich.

Kondratskyi A., Yassine M., Slomianny C., Kondratska K., Gordienko D., Dewailly E., Lehen'kyi V., Skryma R, & Prevarskaya N. (2014). Identification of ML-9 as a lysosomotropic agent targeting autophagy and cell death. Cell Death & Disease, 5(4), e1193-.

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Western Blot Analysis of LC3B and β-Actin

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Cells were washed with ice-cold PBS and then solubilized in a buffer containing 50mM Tris-HCl (pH 7.4), 150mM NaCl, 1% NP-40, 0.5% sodium deoxycholate, 0.1% SDS, 1mM EGTA, protease and phosphatase inhibitor cocktail (Roche, Mannheim, Germany) and cleared by centrifugation at 10000 g. Total protein was separated on a polyacrylamide gel and transferred to a polyvinylidene difluoride (PVDF) membrane (Bio-Rad, Hercules, CA). The membranes were blocked with 5% nonfat dry milk (Bio-Rad) for 1 h and then incubated with primary antibodies. The primary antibodies used were rabbit anti-LC3B (L7543, 1:1000, Sigma-Aldrich), and goat anti-β-actin (sc-1616, 1:500; Santa Cruz Biotechnology). After washing, the membranes were incubated in HRP-conjugated secondary antibodies. The protein signals were detected using the Clarity Western ECL Substrate (Bio-Rad).

Tanaka S., Nagashima H., Uotani T., Graham D.Y, & Yamaoka Y. (2017). Autophagy-related genes in Helicobacter pylori infection. Helicobacter, 22(3), 10.1111/hel.12376.

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Antibody Validation for Protein Analysis

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For immunofluorescence and Western blotting, the following primary antibodies were used: rabbit anti-p62 [P0067] (1:2,000 for Western blot; 1:100 for immunofluorescence), mouse anti-β-actin [A1978] (1:10,000), rabbit anti-LC3B [L7543] (1:1,000), rabbit anti-CCM2 [HPA020273] (1:1,000), and rabbit anti-AMBRA1 [PRS4555] (1:1,000) from Sigma-Aldrich; rabbit anti-GAPDH [#2118] (1:5,000), rabbit anti-mTOR [#2983] (1:1,000), rabbit anti-phospho-mTOR (Ser 2448) [#5536] (1:1,000), rabbit anti-p70 S6 Kinase [#9202] (1:1,000), rabbit anti-phospho-p70 S6 Kinase (Ser 371) [#9208] (1:1,000), rabbit anti-4E-BP1 [#9644] (1:1,000), rabbit anti-phospho-4E-BP1 (Thr 37/46) [#2855] (1:1,000), rabbit anti-ULK1 [#8054] (1:1,000), and rabbit anti-phospho-ULK1 (Ser 757) [#6888] (1:500) from Cell Signaling; rabbit anti-phospho-AMBRA1 (Ser 52) [#ABC80] (1:1,000) from Millipore; rabbit anti-alpha-SMA [NB 600-531] (1:1,000) from NovusBio; mouse anti-N-cadherin [33–3900] (1:500) from Invitrogen; goat anti-CD31/Pecam-1 [sc-1506] (1:1,000), mouse anti-LAMIN A/C [sc-7292] (1:1,000), and mouse anti-VE-cadherin [sc-9989] (1:500) from Santa Cruz Biotechnology; and rabbit anti-KRIT1 (1:500) from S.F. Retta.

Marchi S., Corricelli M., Trapani E., Bravi L., Pittaro A., Delle Monache S., Ferroni L., Patergnani S., Missiroli S., Goitre L., Trabalzini L., Rimessi A., Giorgi C., Zavan B., Cassoni P., Dejana E., Retta S.F, & Pinton P. (2015). Defective autophagy is a key feature of cerebral cavernous malformations. EMBO Molecular Medicine, 7(11), 1403-1417.

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