Cell migration assay was evaluated by using wound healing and Transwell membrane chambers (8 µm pore size; Corning Inc., New York, NY, USA). The wound healing assay was performed as previously described (16 (link)). Briefly, the cells were seeded in 12-well plates and treated with ISO at 90% confluence and normal culture medium was added to the control cells. For Transwell membrane chambers, cells were seeded into the upper chambers and cultured with or without ISO for 36 h. For invasion assay, top chambers coated with Matrigel (BD Biosciences, Franklin Lakes, NJ, USA) at a concentration of 1.37 mg/ml. Upper chambers were fixed in 4% paraformaldehyde at room temperature for 30 min, stained using 0.1% crystal violet at room temperature for 5 min, then counted using a Axioskop 40 (Carl Zeiss AG) microscope.
Transwell membrane chambers
Manufactured by BD
Sourced in United States
Transwell membrane chambers are a laboratory equipment used for cell culture and cell-based assays. They consist of a porous membrane that separates two compartments, allowing for the study of cell migration, permeability, and other cellular processes.
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Lab products found in correlation
2 protocols using transwell membrane chambers
1
Cell Migration and Invasion Assay
2
Wound Healing & Cell Migration Assays
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In wound healing assay, a sterile pipet tip was used to create wounds in confluent cells and then to remove free-floating cells and debris. After 48 h, wound healing was documented and visualized. 24-well transwell chambers (Corning) were used to perform cell-migration assays. 3×104 cells were seeded in the upper chamber and cultured with serum-free medium for 24 h, and then the number of cells in the lower chamber was counted. BD Matrigel (1:5) was added and incubated for 4 h in the transwell membrane chambers for cell-invasion assays. 8×104 cells were seeded in the upper chamber and cultured with serum-free medium for 48 h and then the number of cells in the lower chamber was counted.
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