Avance 3 nano bay 400 mhz ft nmr spectrophotometer

Melting points were recorded on a Stuart SMP10 digital melting point apparatus and were uncorrected. Infrared (IR) spectra were recorded as KBr disks using a Shimadzu FT-IR 8400S infrared spectrophotometer. Mass spectral data are given as m/z (intensity %). ¹H NMR spectra were recorded on either on a Varian Mercury VX-300 MHz spectrophotometer or Bruker AVANCE III Nano Bay 400 MHz FT-NMR spectrophotometer. ¹³C NMR spectra were run at 100 MHz in deuterated dimethylsulfoxide (DMSO-d₆) on Bruker AVANCE III Nano Bay 400 MHz FT-NMR spectrophotometer. Chemical shifts are expressed in δ values (ppm) using the solvent peak as internal standard. All coupling constant (J) values are given in Hz. The abbreviations used are as follows: s, singlet; d, doublet; t, triplet; m, multiplet. Elemental microanalyses were carried out at the Regional Center for Mycology and Biotechnology, Al-Azhar University, Egypt. Reaction were routinely monitored by Thin Layer Chromatography (TLC) on silica gel precoated F254 Merck plates. Unless otherwise noted, all solvents and reagents were commercially available and used without further purification. Compounds 2²² , 4a–f²³ (link)^,24 , 6a–f²³ (link)^,24 , 7a²⁵ , 8a,b^{26 ,27} and 9a²⁸ were prepared according to the reported procedures.