Institutional Review Board approval was obtained for all the procedures. Following the written informed consent of parents, fresh human umbilical cords were obtained after birth from the Second Affiliated Hospital of Jinan University. hUCMSCs were isolated and cultured as previously described (16 (link)). The cells were cultured in Dulbecco's modified Eagle's medium (DMEM)/F12 (Gibco, Grand Island, NY, USA) with 10% fetal bovine serum (FBS), 100 U/ml penicillin G and 100 mg/ml streptomycin and maintained for 3–5 days in a humidified incubator at 37°C with 5% CO2. Flow cytometry on an Altra (Beckman Coulter, Fullerton, CA, USA) was performed to analyze characteristic MSC markers including CD29, CD34, CD90F, CD45, CD106 and HLA-DR (eBioscience, Inc., San Diego, CA, USA). hUCMSCs were also characterized by differentiation toward adipogenic and osteogenic lineages using previously described protocols (Alizarin red and Oil Red O assay) by commercial kits (Cyagen Biosciences Inc., Santa Clara, CA, USA).
Cd90f
Manufactured by Thermo Fisher Scientific
Sourced in United States
The CD90F is a lab equipment product offered by Thermo Fisher Scientific. It is designed to detect and measure the presence of CD90, a cell surface protein, in biological samples. The CD90F provides a reliable and precise method for researchers to analyze and quantify CD90-expressing cells, which can be useful in various areas of study, such as stem cell research and immunology.
Automatically generated - may contain errors
Lab products found in correlation
Cd106, by Thermo Fisher Scientific (1 mentions)
Hla dr, by Thermo Fisher Scientific (1 mentions)
Dmem f12, by Thermo Fisher Scientific (1 mentions)
Alizarin red, by Cyagen (1 mentions)
Oil red o, by Cyagen (1 mentions)
Percp cy5, by BioLegend (1 mentions)
Cd146, by BioLegend (1 mentions)
Cd105, by Thermo Fisher Scientific (1 mentions)
Facsverse, by BD (1 mentions)
2 protocols using cd90f
1
Isolation and Characterization of hUCMSCs
2
Phenotypic Analysis of Adipose-Derived Stem Cells
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Surface markers of ADSCs were analyzed using a FACSVerse and BD FACSuite software version 10 (BD Biosciences, Tokyo, Japan), as described previously. 9, 16, 17 Briefly, ADSCs were collected by trypsinization and washed twice with phosphate-buffered saline. Five microliters of antibodies against CD31 (PE conjugated; e-Bioscience, San Diego, Calif), CD34 (FITC-conjugated; e-Bioscience), CD45 (PerCP/Cy5.5 conjugated; BioLegend, San Diego, Calif), CD90f (APC conjugated; e-Bioscience), CD105 (APC conjugated; e-Bioscience), or CD146 (APC conjugated; Bio Legend) were added to the cell pellets after addition of 1300 μL FACS buffer. The cells were incubated at room temperature (RT) for 30 minutes in the dark, washed with FACS buffer, and then analyzed by the FACSVerse.
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