Soluble Aβ42 oligomers were prepared as previously described (Stine et al., 2003 (link)). 1mg of lyophilized human Ab42 (Anaspec, Fremont, CA) was dissolved in 1mL of 1,1,1,3,3,3-hexafluoro-2-propanol (HFIP) (Sigma, St. Louis, MO) to prevent aggregation, portioned into 10μg aliquots, air-dried and stored at −80°C. For use in experiments, an aliquot was thawed at room temperature, and then dissolved in DMSO and PBS to make a 100μM solution. The solution was incubated for 16 hours at 4°C and then diluted to a final concentration for use in experiments. The following antagonists were used in this study: 50nM α-Bungarotoxin (αBTx) (Alomone labs, Jerusalem, Israel), 1μM Dihydro-β-erythroidine hydrobromide (DHβE) (Tocris Bioscience, Bristol, UK), and 3μM α-Conotoxin AuIB (Alomone labs). The following agonists were used in this study: 25nM Muscimol (MP Biomedicals, Santa Ana, CA), 1μM PNU-120596 (Alomone labs), 2μM RJR-2403 Oxalate (Alomone labs), and 1μM Carbamoylcholine chloride (carbachol) (Tocris Bioscience).
Pnu 120596
Manufactured by Alomone
PNU-120596 is a selective positive allosteric modulator (PAM) of the α7 nicotinic acetylcholine receptor (α7 nAChR). It enhances the function of the α7 nAChR, which is involved in various physiological processes.
Automatically generated - may contain errors
Lab products found in correlation
Rjr 2403 oxalate, by Alomone (3 mentions)
Carbamoylcholine chloride carbachol, by Bio-Techne (3 mentions)
Scrambled aβ42, by AnaSpec (2 mentions)
Ns 3861, by Bio-Techne (2 mentions)
Muscimol, by MP Biomedicals (1 mentions)
Dihydro β erythroidine hydrobromide dhβe, by Bio-Techne (1 mentions)
Hexafluoro 2 propanol hfip, by Merck Group (1 mentions)
Hexafluoro 2 propanol hfip, by Thermo Fisher Scientific (1 mentions)
3 protocols using pnu 120596
1
Soluble Aβ42 oligomer preparation and use
2
Preparation and Application of Amyloid-beta Oligomers
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Soluble Aβ42 oligomers or soluble scrambled Aβ42 oligomers were prepared as described (32 (link), 104 (link)). One milligram of lyophilized human Aβ42 (Anaspec) or scrambled Aβ42 (Anaspec) was dissolved in 1 ml of 1,1,1,3,3,3-hexafluoro-2-propanol (Fisher Scientific) to prevent aggregation, portioned into 10-μg aliquots, air dried, and stored at −80 °C. For use in experiments, an aliquot was thawed at room temperature and then dissolved in 100% dimethyl sulfoxide, then diluted into PBS to make a 100 μM solution. The solution was incubated for 16 h at 4 °C and then diluted to a final concentration for use in experiments. The following agonists were used in this study: 1 μM PNU-120596 (Alomone Labs), 2 μM RJR-2403 Oxalate (Alomone labs), 1 μM NS-3861 (Tocris Bioscience), and 1 μM Carbamoylcholine chloride (carbachol) (Tocris Bioscience). Aβcore (YEVHHQ) and inactive Aβcore (SEVAAQ) peptides were prepared as described (43 (link)).
3
Oligomeric Aβ42 Preparation and Agonist Treatment
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Soluble Aβ42 oligomers or soluble scrambled Aβ42 oligomers were prepared as previously described (32) . One mg of lyophilized human Ab42 (Anaspec) or scrambled Aβ42 (Anaspec) was dissolved in 1mL of 1,1,1,3,3,3-hexafluoro-2-propanol (HFIP) (Fisher Scientific) to prevent aggregation, portioned into 10μg aliquots, air-dried and stored at -80°C. For use in experiments, an aliquot was thawed at room temperature, and then dissolved in 100% DMSO then diluted into PBS to make a 100μM solution. The solution was incubated for 16 hours at 4°C and then diluted to a final concentration for use in experiments. The following agonists were used in this study: 1μM PNU-120596 (Alomone labs), 2μM RJR-2403 Oxalate (Alomone labs), 1μM NS-3861 (Tocris Bioscience), and 1μM Carbamoylcholine chloride (carbachol) (Tocris Bioscience). Aβcore (YEVHHQ) and inactive Aβcore (SEVAAQ) peptides were prepared as described previously (43) .
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