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V 530 double beam uv vis spectrophotometer

Manufactured by Jasco
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Sourced in Japan

The V-530 double beam UV–VIS spectrophotometer is a laboratory instrument used to measure the absorption or transmission of light in a sample over a range of ultraviolet and visible wavelengths. It utilizes a dual-beam optical system to compare the light intensity between a reference and a sample.

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Lab products found in correlation

Whatman filter paper, by Cytiva (1 mentions) Whatman, by Cytiva (1 mentions) Vertex 70 spectrophotometer, by Bruker (1 mentions) Hightech ht7700 transmission electron microscope, by Hitachi (1 mentions)

Spelling variants of this product

V-530 (208 citations) V-530 spectrophotometer (87 citations) UV-Vis spectrophotometer (66 citations) UV spectrophotometer (11 citations) UV/VIS V-530 (10 citations) V-530 double beam spectrophotometer (3 citations) UV-530 spectrophotometer (3 citations) UV-530 (3 citations) Double beam spectrophotometer (2 citations)

4 protocols using v 530 double beam uv vis spectrophotometer

1

Spectrophotometric Determination of Drug Encapsulation Efficiency

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The formulated ACZ-NVs were separated by cooling ultracentrifugation at 12,000 rpm and 4 °C for 15 min. The pellet was dissolved using absolute ethanol and the absorbance was measured spectrophotometrically (JASCO V-530 double beam UV–VIS spectrophotometer connected to a computer loaded with Spectra Manager Program, Japan) at maximum wavelength 265 nm and then the amount of the ACZ drug was calculated according to the following equation:
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\begin{document}
$${\rm{\% EE = }}{{{\rm{Amount\ of\ drug\ actually\ present }}} \over {{\rm{ Total\ amount\ of\ drug\ added}}}} \times 100$$
\end{document}%EE=Amount of drug actually presentTotal amount of drug added×100
Abdel-Rashid R.S., Helal D.A., Omar M.M, & El Sisi A.M. (2019). Nanogel loaded with surfactant based nanovesicles for enhanced ocular delivery of acetazolamide. International Journal of Nanomedicine, 14, 2973-2983.
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2

Spectrophotometric Analysis of Clonidine

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Chemicals and reagentsMethods used for ion-pair formation:Preparation of stock standard solution100μg/mL:10 mg of clonidine hydrochloride was dissolved in 3 mL methanol and completed to 100 mL with distilled water.
Working solutionsContaining from 0.5-7.5 μg/mL clonidine hydrochloride were obtained by diluting the stock solution with distilled water.
Preparation of sample solutions:
Twenty tablets from each sample product were weighed. Their average weight was calculated and then they were finely powdered in a glass mortar. Two quantities of powder, each equivalent to 30 mg clonidine hydrochloride, were weighed and transferred into a 100 mL volumetric flask. 10 mL methanol and 20 mL distilled water were added to both flasks and stirred for 10 min to dissolve the drug. The solutions were filtered through Whatman filter paper and completed to 100 mL with distilled water. The sample solutions were marked as Sample 1 and Sample 2. The procedure was continued as described on ion-pair formation procedure.
InstrumentA Jasco V 530 double beam UV-Vis spectrophotometer was used. All the measurements were made in 1.0 cm quartz cells at a scan speed of 1000 nm min-1 and scan range of 400-600 nm, fixed slit width of 2 nm.
, & Corciova A. (2016). Validated Colorimetric Assay of Clonidine Hydrochloride from Pharmaceutical Preparations. Iranian Journal of Pharmaceutical Research : IJPR, 15(1), 149-156.
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3

UV-Vis Spectroscopy and Photographic Instrumentation

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UV-vis spectra were recorded in the range of 400–800 nm using a Jasco V-530 UV/vis double beam spectrophotometer (Tokyo, Japan). A DAIHAN hot plate magnetic stirrer (Batam, Indonesia) was used. All the materials were weighed using a Sartorius BP221S 4-digit analytical balance (Göttingen, Germany). A smartphone equipped with a 12-megapixel camera and the Android 13.0 operating system (Samsung Galaxy note 10 lite, Vietnam) was used for photo acquisition.
El-Hassanein A.M., Mansour F.R., Hammad S.F, & Abdella A.A. (2024). Simple colorimetric paper-based test strip for point-of-use quality testing of ethanol-based hand sanitizers. RSC Advances, 14(12), 8188-8194.
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4

Biosynthesis and Characterization of Silver Nanoparticles

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The formation of AgNPs was observed through the color change of Equisetum extracts after being mixed with AgNO3. The UV-Vis spectra were recorded in the 300–600 nm range to distinguish the maximum surface plasmon resonance (SPR), using a Jasco V-530 UV-Vis double beam spectrophotometer for this determination. The FTIR spectra were obtained with a Bruker Vertex 70 spectrophotometer, by potassium bromide tableting, over a scan interval of 4000–310 cm−1. The EDX qualitative analysis was performed using a Quanta 200 Environmental Scanning Electron Microscope (ESEM) with EDX. The used EDX detector allowed rapid determination of the elementary composition of nanoparticles. The DLS measurements were performed using a Delsa Nano Submicron Particle Size Analyzer (Beckman Colter) that provided the average diameter of nanoparticles and the Zeta potential value. Additionally, TEM analysis was performed using a Hitachi High-Tech HT 7700 Transmission Electron Microscope.
Moreover, prior to the purification and characterization of AgNPs, the supernatant and the initial plant extract were analyzed taking into account the phenolic content using the modified spectrophotometric Folin-Ciocâlteu method [26 (link)]. Gallic acid was used as the standard and the results were expressed in mg gallic acid equivalents (GAE)/mL sample.
Batir-Marin D., Mircea C., Boev M., Burlec A.F., Corciova A., Fifere A., Iacobescu A., Cioanca O., Verestiuc L, & Hancianu M. (2021). In Vitro Antioxidant, Antitumor and Photocatalytic Activities of Silver Nanoparticles Synthesized Using Equisetum Species: A Green Approach. Molecules, 26(23), 7325.
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