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3h glibenclamide

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[3H]-glibenclamide is a radiolabeled compound used in research applications. It is a tritium-labeled version of the drug glibenclamide, which is a sulfonylurea commonly used to treat type 2 diabetes. [3H]-glibenclamide can be used in various experimental techniques, such as receptor-binding assays, to study the pharmacological properties and interactions of glibenclamide.

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Lab products found in correlation

Glimepiride, by Merck Group (2 mentions) Rotiszint eco plus, by Carl Roth (2 mentions) Gf c filters, by Brandel Inc (2 mentions) Mwxr 96 ti harvester, by Brandel Inc (2 mentions) Packard microbeta scintillation counter, by PerkinElmer (2 mentions)

2 protocols using 3h glibenclamide

1

Radioligand Binding Assay for SUR1 in HEK293t Cells

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SUR1-expressing HEK293t cells were harvested and washed twice in assaying buffer containing in mM: 119 NaCl, 4.7 KCl, mM CaCl2, 1.2 KH2PO4, 1.2 MgSO4, 5 NaHCO3, and 20 HEPES, pH 7.4. In a 96-well plate, ~200,000 cells/well were incubated for 50 min with [3H]-glibenclamide (PerkinElmer) and different concentrations of glimepiride (Sigma-Aldrich) or JB253. Incubation was terminated by rapid filtration through Whatman GF/C filters by means of a Brandel MWXR-96 TI harvester and filters were washed 3 times with ice-cold assay buffer. Radioactivity was counted 6 h after cell and filter lysis in 200 μL Rotiszint EcoPlus (Roth) using a Packard microbeta scintillation counter (PerkinElmer).
Broichhagen J., Schönberger M., Cork S.C., Frank J.A., Marchetti P., Bugliani M., Shapiro A.M., Trapp S., Rutter G.A., Hodson D.J, & Trauner D. (2014). Optical control of insulin release using a photoswitchable sulfonylurea. Nature Communications, 5, 5116.
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2

Radioligand Binding Assay for SUR1 Receptors

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SUR1-expressing HEK293t cells were harvested and washed twice in assaying buffer containing in mM: 119 NaCl, 4.7 KCl, mM CaCl2, 1.2 KH2PO4, 1.2 MgSO4, 5 NaHCO3 and 20 HEPES, pH 7.4. In a 96-well plate, ~200,000 cells per well were incubated for 50 min with [3H]-glibenclamide (PerkinElmer) and different concentrations of glimepiride (Sigma-Aldrich) or JB253. Incubation was terminated by rapid filtration through Whatman GF/C filters by means of a Brandel MWXR-96 TI harvester and filters were washed three times with ice-cold assay buffer. Radioactivity was counted 6 h after cell and filter lysis in 200 μl Rotiszint EcoPlus (Roth) using a Packard microbeta scintillation counter (PerkinElmer).
Broichhagen J., Schönberger M., Cork S.C., Frank J.A., Marchetti P., Bugliani M., Shapiro A.M., Trapp S., Rutter G.A., Hodson D.J, & Trauner D. (2014). Optical control of insulin release using a photoswitchable sulfonylurea. Nature Communications, 5, 5116.
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