Lysosenser yellow blue dextran

Rapamycin (Rapa), coumarin-6 (Cou-6), and okadaic acid (OA) were purchased from Sigma–Aldrich Inc. (St.Louis, MO, USA). Gypenoside XVII (GP-17) was attained from Push Biotechnology Co., Ltd. (Chengdu, China). RP-1 (CAPDTKTQ) and Aβ_1–42 were synthesized by Guotai Biotechnology Co., Ltd. (Hefei, China) and Chinese Peptide Co., Ltd. (Hangzhou, China), respectively. 1,1′-Dioctadecyl-3,3,3′,3′-tetramethylindocarbocyanine perchlorate (DiI), 3,3′-dioctadecyloxacarbocyanine perchlorate (DiO), Cell Counting Kit-8 (CCK-8), Total ROS assay kit and BCA Protein Assay Kit were purchased from Beyotime Biotechnology Co., Ltd. (Shanghai, China). Amyloid-β1–40 Human ELISA Kit and Amyloid-β1–42 Human ELISA Kit were purchased from Elabscience Biotechnology Co., Ltd. (Shanghai, China). Lysotracker Red DND-99 and Lysosenser Yellow/Blue dextran were purchased from Thermo Fisher Scientific, Inc. (Eugene, OR, USA). The mouse brain microvascular endothelial cells (bEnd.3), mouse hippocampal neuronal cells (HT22), and murine neonatal microglial cells (BV2) were obtained from the Chinese Academy of Sciences Cell Bank (Shanghai, China). All chemicals used were analytical or reagent grade.

Rapamycin (Rapa), coumarin-6 (Cou-6), and okadaic acid (OA) were purchased from Sigma–Aldrich Inc. (St.Louis, MO, USA). Gypenoside XVII (GP-17) was attained from Push Biotechnology Co., Ltd. (Chengdu, China). RP-1 (CAPDTKTQ) and Aβ_1–42 were synthesized by Guotai Biotechnology Co., Ltd. (Hefei, China) and Chinese Peptide Co., Ltd. (Hangzhou, China), respectively. 1,1′-Dioctadecyl-3,3,3′,3′-tetramethylindocarbocyanine perchlorate (DiI), 3,3′-dioctadecyloxacarbocyanine perchlorate (DiO), Cell Counting Kit-8 (CCK-8), Total ROS assay kit and BCA Protein Assay Kit were purchased from Beyotime Biotechnology Co., Ltd. (Shanghai, China). Amyloid-β1–40 Human ELISA Kit and Amyloid-β1–42 Human ELISA Kit were purchased from Elabscience Biotechnology Co., Ltd. (Shanghai, China). Lysotracker Red DND-99 and Lysosenser Yellow/Blue dextran were purchased from Thermo Fisher Scientific, Inc. (Eugene, OR, USA). The mouse brain microvascular endothelial cells (bEnd.3), mouse hippocampal neuronal cells (HT22), and murine neonatal microglial cells (BV2) were obtained from the Chinese Academy of Sciences Cell Bank (Shanghai, China). All chemicals used were analytical or reagent grade.