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Akt polyclonal antibody

Manufactured by Proteintech
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Sourced in China, United States

The AKT polyclonal antibody is a laboratory reagent used for protein detection and analysis. It is a protein-specific antibody that binds to the AKT protein, which is involved in various cellular processes. The antibody can be used in techniques such as western blotting, immunoprecipitation, and immunohistochemistry to identify and quantify the AKT protein in biological samples.

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Lab products found in correlation

Bca method, by Beyotime (1 mentions) Sds page, by Beyotime (1 mentions) Nfκb p65 polyclonal antibody, by Proteintech (1 mentions) Cd81 monoclonal antibody, by Proteintech (1 mentions) Goat anti mouse igg h l, by Proteintech (1 mentions) Goat anti rabbit igg h l, by Proteintech (1 mentions) Phospho mtor ser2448, by Cell Signaling Technology (1 mentions) Phospho p70 s6 kinase thr389, by Cell Signaling Technology (1 mentions) Phospho akt ser473, by Cell Signaling Technology (1 mentions) Phospho akt thr308, by Cell Signaling Technology (1 mentions) Ha tag antibody, by ABclonal (1 mentions) Erk1 2 monoclonal antibody, by Cell Signaling Technology (1 mentions) Phospho mdm2 ser166, by Cell Signaling Technology (1 mentions) β actin rabbit mab, by ABclonal (1 mentions) Phospho pdk1 ser241, by Cell Signaling Technology (1 mentions) Phospho p44 42 mapk erk1 2 thr202 tyr204, by Cell Signaling Technology (1 mentions) Anti gapdh, by Sangon (1 mentions) P70s6k, by Proteintech (1 mentions)

Spelling variants of this product

Akt rabbit polyclonal antibody (5 citations) AKT antibody (3 citations)

3 protocols using akt polyclonal antibody

1

Protein Analysis of Cellular Vesicles

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The proteins of suspensions OFs, exosomes, and tissues were obtained and quantified by the BCA method (Beyotime, Shanghai, China). Protein lysates were transferred to the PVDF membrane by 8% SDS-PAGE (Beyotime Biotechnology, China). The PVDF membrane was cleaned with TBST and sealed with 5% skim milk powder for 2 h. The cut film was incubated overnight in the primary antibody dilution solution and 2 h in the secondary antibody dilution solution. Finally, the film was scanned. The main antibodies used included HRP-binding GAPDH monoclonal antibody (ProteinTech, Wuhan, China), AKT polyclonal antibody (ProteinTech, China), NFκB P65 polyclonal antibody (ProteinTech, China), Rabbit Anti-phosphorylated NFκB P65 (Ser276) Antibody (Bioss, Beijing, China), Rabbit Anti-phosphorylated AKT (Ser473) antibody (Bioss, China), IL-1α polyclonal antibody (ProteinTech, China), ICAM1 polyclonal antibody (ProteinTech, China), CD9 monoclonal antibody (ProteinTech, China), TSG101 polyclonal antibody (ProteinTech, China), CD81 monoclonal antibody (ProteinTech, China), Goat Anti-mouse IgG (H+L) (ProteinTech, China), Goat anti-rabbit IgG (H+L) (ProteinTech, China), and glucocorticoid receptor polyclonal antibody (ProteinTech, China).
Sun J., Wei J., Zhang Y., Li J., Li J., Yan J., Guo M., Han J, & Qiao H. (2022). Plasma Exosomes Transfer miR-885-3p Targeting the AKT/NFκB Signaling Pathway to Improve the Sensitivity of Intravenous Glucocorticoid Therapy Against Graves Ophthalmopathy. Frontiers in Immunology, 13, 819680.
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2

Protein Expression Analysis by Western Blot

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Cells extracts were prepared and analyzed as previously described [16 (link)]. Western blotting was performed by anti-ZKSCAN1 (Bioss, P17029), FBXW7 Rabbit Polyclonal Antibody (ABclonal, A5872), mTOR Mouse Monoclonal antibody (Proteintech, 66,888–1-lg), β-Actin Rabbit mAb (ABclonal, AC026), anti-His Mouse Monoclonal antibody (ABclonal, AE003), HA-tag antibody (ABclonal, AE008), anti DDDDK-Tag Mouse Monoclonal antibody (ABclonal, AE005), anti-GAPDH (Sangon Biotech, D110016), AKT Polyclonal Antibody (Proteintech, 51,077–1-AP), Phospho-Akt (Ser473) (Cell Signaling Technology, 4060), Phospho-Akt (Thr308) (Cell Signaling Technology, 13,038), Phospho-p44/42 MAPK (Erk1/2) (Thr202/Tyr204) (Cell Signaling Technology, 4370), ERK1/2 Monoclonal Antibody (67170–1-Ig), Phospho-mTOR (Ser2448) (Cell Signaling Technology, 5536), Phospho-PDK1 (Ser241) (Cell Signaling Technology, 3438), Phospho-MDM2 (Ser166) (Cell Signaling Technology, 3521), Phospho-p70 (S6) Kinase (Thr389) (Cell Signaling Technology, 97,596), MDM2 (Proteintech, 66,511–1-Ig), PDK1 (Proteintech, 18,262–1-AP), P70 (S6K) (Proteintech, 14,485–1-AP), Biotinylated Rabbit anti-Goat IgG (ZSGB-BIO, ZB-2050) and Biotinylated Mouse anti-Goat IgG (ZSGB-BIO, ZB-2020) antibodies. The antigen-antibody complexes were visualized by chemiluminescence.
Song R., Ma S., Xu J., Ren X., Guo P., Liu H., Li P., Yin F., Liu M., Wang Q., Yu L., Liu J., Duan B., Rahman N.A., Wołczyński S., Li G, & Li X. (2023). A novel polypeptide encoded by the circular RNA ZKSCAN1 suppresses HCC via degradation of mTOR. Molecular Cancer, 22, 16.
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3

Traditional Chinese Medicine Extraction

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Juju (Herba CichoriiIntybi) was purchased from Xinjiang Minhai Traditional Chinese Medicine Decoction Pieces Co., Ltd. (Aletai, China). Fuling (Poria) and Tufuling (Rhizoma Smilacis Chinae) were purchased from Beijing BencaoFangyuan Pharmaceutical Group Co., Ltd. (Beijing, China). The appropriate amount of medicine was weighed in proportion and the ratio of material to liquid was 10 times the mass of deionized water. The extracts were prepared with boiling water for 1hr and extracted twice. Then a rotary evaporator was used to concentrate the solution after filtering and deliquated to different concentrations with purified water.
The Dextran sulfate was obtained from Shanghai Yuanye Biotechnology Co., Ltd. (Shanghai, China). Enteric coated mesalazine tablets were purchased from Jiamusi Luling Pharmaceutical Co., Ltd. (Jiamusi, China). Enzyme linked immunosorbent assay kits for tumor necrosis factor (TNF-α), interleukin-6 (IL-6), and interleukin-1β (IL-1β) assays were obtained from Shanghai Jiangle Biotechnology Co., Ltd. (Shanghai, China). The CD3 Polyclonal Antibody, AKT Polyclonal antibody, and Phospho-AKT (Ser473) Monoclonal antibody were supported by Proteintech Group, Inc.(Rosemont, IL, USA), and Foxp3 (D6O8R) Rabbit mAb was supported by CST Co., Ltd. (Danvers, MA, USA).
Mengzhen C., Yu W., Zhijian L., Jintao L., Xiaomeng Z, & Bing Z. (2024). Investigation of the active ingredients and mechanism of Shuangling extract in dextran sulfate sodium salt induced ulcerative colitis mice based on network pharmacology and experimental verification. Journal of traditional Chinese medicine = Chung i tsa chih ying wen pan, 44(3).
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