Ywahe 14 3 3ε

Azido-tagged biotin with or without a photocleavable linker (biotin-UV-azide and biotin-azide) were provided by Professor Ned A. Porter ^{6 (link)} (Supplementary Figure 10). The synthesis of these reagents is described in the Supplementary Material of Reference 6. DYn-2 was purchased from Cayman chemicals. DYn-2-d6 was synthesized as described in the Supplementary Methods. Strong cation exchange (SCX) spin columns were purchased from Nest group. Streptavidin sepharose was purchased from GE Healthcare Life Sciences. HPLC-grade water, acetonitrile (ACN), and methanol were purchased from J.T.Baker. Other chemicals and reagents were obtained from Sigma unless otherwise indicated. Antibodies against ACLY (ab40793, diluted at 1:5000), ACTN (ab6276, diluted at 1:5000), CFL1 (ab42824, diluted at 1:1000), EEF1G (ab72368, diluted at 1:5000), MAPK1/ERK2 (ab124362, diluted at 1:1000), RPL18 (ab166711, diluted at 1:1000), SIRT6 (ab88494, diluted at 1:2500), TUBB (ab7792, diluted at 1:5000), PRDX-SO_2/3H (ab16830, diluted at 1:2000), PRDX1 (ab15571, diluted at 1:2000), and PRDX2 (ab15572, diluted at 1:2000) were purchased from Abcam. Antibodies against HSP90 (CS#4874S, diluted at 1:1000) and YWAHE/14-3-3ε (CS#9635S, diluted at 1:1000) were purchased from Cell Signaling Technology.