E bc k227 s

Human tissue microarray slides were deparaffinized as described previously (13 (link)). For mice tissue staining, mice livers were isolated from the indicated male mice, rinsed in ice-cold PBS, fixed in 4% polyformaldehyde for 48 h at 4°C, and then dehydrated, embedded in paraffin, and sectioned. Tissue sections (4 μm) were used for staining. Slides were then incubated with the α-SETD3 or α-PLK1 antibody at 1:1,000, respectively, followed by biotin-conjugated goat anti-rabbit IgG antibody or goat anti-mouse IgG antibody at 1:10,000, respectively. Detections were performed using a 3,3′-diaminobenzidine (DAB) reagent (Cat. E-BC-K227-S, Elabscience, Wuhan, China), and nuclei were stained with hematoxylin before examination by a microscope. At least four mice per group were used for assay. The intensity score was determined by evaluating staining intensity of positive staining as described previously (13 (link)).