The following mAbs were used: FITC-anti CD20, PE-Cy7-anti-CD27, APC-anti-CD10, PE-anti-CD5, APC-anti-CD38, BV421-anti-CD21, biotin anti-CD44, BV711-anti-CD19, FITC anti-CCR7 (CD197), PE anti-CXCR4 (CD184), biotin anti-CXCR5 (CD185), PE-anti-IgM, BV605-anti-IgG, biotin-anti-IgA, PE-anti-Bcl-2, PE-anti-Blimp-1, Streptavidin-BV605, and Streptavidin-PerCP-Cy5.5 (all from Becton Dickinson); PE-anti-CD23 and FITC-anti-CD38 (eBioscience); and biotin-anti-IgD (Southern Biotech).
Biotin anti igd
Manufactured by Southern Biotech
Sourced in United States
Biotin-anti-IgD is a laboratory reagent used in immunoassays and other immunological techniques. It is a conjugate of biotin, a small molecule with high affinity for the protein streptavidin, and an antibody specific for the IgD class of immunoglobulins. This reagent can be utilized to detect and quantify IgD in biological samples.
Automatically generated - may contain errors
Lab products found in correlation
Streptavidin percp cy5, by BD (1 mentions)
Apc anti cd10, by BD (1 mentions)
Bv605 anti igg, by BD (1 mentions)
Biotin anti iga, by BD (1 mentions)
Anti igm fitc, by BD (1 mentions)
Pe anti cd138, by BD (1 mentions)
Anti igd fitc, by BD (1 mentions)
Anti cd16 cd32 antibody, by BD (1 mentions)
Canto 2, by BD (1 mentions)
Facsdiva, by BD (1 mentions)
2 protocols using biotin anti igd
1
Multi-marker Immunophenotyping of B-cells
2
Multiparametric Flow Cytometry of Splenic B Cells
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The surface expression level of CD23 on splenic B cells was analyzed by flow cytometry. B cells were pre-incubated with anti-CD16/CD32 antibody (BD Biosciences, San Jose, CA, USA, Cat. No 553142) to block FcγR, and following by PerCP-Cy5.5-anti-B220 (BD Biosciences, Cat. No 552771), PE-anti-CD138 (BD Biosciences, Cat. No 553714), PE-Cy7-anti-IgM (BD Biosciences, Cat. No 552867), biotin-anti-IgD (Southern Biotech, Birmingham, AL, USA, Cat. No 1120-08), FITC-anti-IgD (BD Biosciences, Cat. No 553439), FITC-anti-IgM (BD Biosciences, Cat. No 553408) and APC-NP19, and FITC-anti-CD23 antibodies (BD Biosciences, Cat. No 553138), plus AF405- streptavidin (Thermo Scientfic, Waltham, MA, USA ,Cat. No S32351). After washing and fixation, cells were analyzed using a flow cytometer (BD CantoII, BD Biosciences). Data were analyzed using FACSDiva (BD Biosciences) and FlowJo (Treestar Inc. Ashland, OR, USA) software.
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