The brain tissues processed for immunofluorescence were viewed with a Leica TCS SP5II confocal laser-scanning microscope equipped with Diode- and Argon-lasers and operated by the Leica “Application Suite Advanced Fluorescence” software package (LASAF) (Leica Microsystems, Wetzlar, Germany). Digital images were processed with Adobe Photoshop CS4 or ImageJ. Only global picture enhancement features (brightness and contrast) were used. The brain tissues processed for histology were viewed with a Nikon Eclipse 90i upright microscope and bright-field optics (Nikon, Amstelveen, Netherlands).
Application suite advanced fluorescence software package lasaf
Manufactured by Leica
Sourced in Germany
The Leica 'Application Suite Advanced Fluorescence' (LASAF) software package is a comprehensive solution for advanced fluorescence microscopy imaging and analysis. It provides a suite of tools and functionalities to support various fluorescence-based experiments and applications.
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Lab products found in correlation
2 protocols using application suite advanced fluorescence software package lasaf
1
Confocal Microscopy of Brain Tissue
2
Multimodal Microscopy for Brain Tissue
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The brain tissues processed for immunofluorescence were viewed with a Leica TCS SP5II confocal laser-scanning microscope equipped with DPSS, Diode- and Argon-lasers and operated by the Leica ‘Application Suite Advanced Fluorescence’ software package (LASAF) (Leica Microsystems, Wetzlar, Germany). Digital images were processed with Adobe Photoshop CS4 or ImageJ. Only global picture enhancement features (brightness and contrast) were used.
The head tissues processed for histology were viewed with a Nikon Eclipse 90i upright microscope and bright-field optics (Nikon, Amstelveen, Netherlands). Serial images using a mounted digital camera (Nikon DS-Fi3) were aligned manually with the 3D-reconstruction software Amira 5.6.0 (FEI Visualization Science Group, Burlington, VT, USA; RRID:SCR_007353 ).
For frontal and sagittal sections, dorsal is always towards the top.
In the figures, the following color-coded abbreviations were used to identify the markers: SYN, synapsin (magenta); AstA, allatostatin (green); 5HT, serotonin (green); NUC, nuclear counter stain (cyan). Colors were chosen according to Color Universal Design for accessibility to colorblind readers.
The head tissues processed for histology were viewed with a Nikon Eclipse 90i upright microscope and bright-field optics (Nikon, Amstelveen, Netherlands). Serial images using a mounted digital camera (Nikon DS-Fi3) were aligned manually with the 3D-reconstruction software Amira 5.6.0 (FEI Visualization Science Group, Burlington, VT, USA; RRID:
For frontal and sagittal sections, dorsal is always towards the top.
In the figures, the following color-coded abbreviations were used to identify the markers: SYN, synapsin (magenta); AstA, allatostatin (green); 5HT, serotonin (green); NUC, nuclear counter stain (cyan). Colors were chosen according to Color Universal Design for accessibility to colorblind readers.
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