Log In Sign up
The largest database of trusted experimental protocols

Abi prism 7700 sequence detection system and software

Manufactured by Thermo Fisher Scientific
Visit Supplier

The ABI PRISM 7700 Sequence Detection System is a real-time PCR instrument designed for quantitative gene expression analysis. It provides real-time monitoring of the amplification of DNA sequences. The system includes the instrument hardware and the accompanying software for data analysis.

Automatically generated - may contain errors

Lab products found in correlation

Taqman gene expression assay kit, by Thermo Fisher Scientific (1 mentions) High capacity rna to cdna kit, by Thermo Fisher Scientific (1 mentions) Primer express software version 1, by Thermo Fisher Scientific (1 mentions)

Close spelling variants of this product

ABI PRISM 7700 Sequence Detection System Instrument and software ABI Prism 7700 Sequence Detection System software ABI Prism 7700 Sequence Detection System ABI PRISM 7700 Sequence Detection ABI PRISM 7700 Sequence System PRISM 7700 Sequence Detection System ABI 7700 Sequence Detection System ABI PRISM 7700 detection system ABI Prism 7700 sequence ABI PRISM 7700 system

2 protocols using abi prism 7700 sequence detection system and software

1

Liver tissue RNA extraction and qPCR analysis

Check if the same lab product or an alternative is used in the 5 most similar protocols
The total RNA was extracted, and cDNA was synthesized from 108 snap-frozen liver tissue samples using TRIzol and High Capacity RNA-to-cDNA kit (Invitrogen, Carlsbad, CA, USA) according to the manufacturer’s instructions. TaqMan Gene Expression Assay kits used in this study were purchased from Applied Biosystems (Foster City, CA, USA); the sequences of their primer/probe sets are summarized in Supplementary Table 2. All experiments were performed in triplicate for each cDNA and the relative expression levels of the target mRNAs were normalized to GAPDH mRNA levels. PCR reactions were performed using gene-specific primers and probes with an ABI PRISM 7700 Sequence Detection System and software (Applied Biosystems) according to the TaqMan protocol. A non-template reaction was included in all experiments as a negative control.
Yoo J.E., Nahm J.H., Kim Y.J., Jeon Y, & Park Y.N. (2022). The dual role of transforming growth factor-beta signatures in human B viral multistep hepatocarcinogenesis: early and late responsive genes. Journal of Liver Cancer, 22(2), 115-124.
+ Open protocol
+ Expand
2

Real-Time PCR for T-cell Receptor Excision Circles

Check if the same lab product or an alternative is used in the 5 most similar protocols
The primers and probe were developed using Primer Express software version 1.0 (Applied Biosystems, Foster City, CA, USA). A forward primer 5’-cacatccctttcaaccatgct-3’ and reverse primer 5’-gccagctgcagggtttagg-3’ together with the probe, 5’-FAM-acgcctctggtttttgtaaaggtgctcact-TAMRA-3’ (Applied Biosystems) were used. Real-time PCR was performed using the ABI Prism® 7700 Sequence Detection System and software (Applied Biosystems). Samples, including negative controls and samples previously analyzed, were analyzed in duplicate. A standard curve, used to quantify TRECs, was created from PCR analysis of serial dilutions of a plasmid containing a signal-joint breakpoint (generously provided by Dr. Daniel Douek) (Sodora et al. 2000 (link); Douek et al. 1998 (link); Douek et al. 2000 (link)).
Farese A.M., Hankey K.G., Cohen M.V, & MacVittie T.J. (2015). Lymphoid and myeloid recovery in rhesus macaques following total body x-irradiation. Health physics, 109(5), 414-426.
+ Open protocol
+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required

Sign up now

Revolutionizing how scientists
search and build protocols!