Ge na934v

Manufactured by GE Healthcare

The GE NA934V is a laboratory equipment designed for nucleic acid analysis. It provides a platform for performing automated nucleic acid extraction and purification. The device utilizes magnetic bead-based technology to isolate nucleic acids from various sample types. This product is intended to assist researchers and clinicians in their analytical workflows, however a detailed description of its intended use is not available.

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Lab products found in correlation

Donkey anti rabbit hrp conjugate, by GE Healthcare (3 mentions) Ap101p, by Merck Group (1 mentions) Infinite m50, by Tecan (1 mentions)

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NA934V (138 citations)

4 protocols using ge na934v

Quantifying CVN Protein Binding

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Recombinantly purified CVN and CVN-DAVEI-L2-3Trp proteins (50 ng) were loaded onto the plate simultaneously in the presence of increasing concentrations of 2G12 (10 000–0.03 nM) and incubated for 2 h followed by three PBS-T washes. Rabbit anti-CVN (Biosyn, Inc.; dilution 1: 3000) was added as a primary antibody followed by two washes with 1× PBS-T. The donkey anti-rabbit HRP conjugate (GE Biosciences, GE NA934V; dilution factor 1: 3000) was used as the secondary antibody, which was detected using an OPD solution. CVN and CVN-DAVEI-L2-3Trp proteins loaded alone (without 2G12 added) were used as positive controls.

Parajuli B., Acharya K., Bach H.C., Parajuli B., Zhang S., Smith AB I.I.I., Abrams C.F, & Chaiken I. (2018). Restricted HIV-1 Env glycan engagement by lectin-reengineered DAVEI protein chimera is sufficient for lytic inactivation of the virus. The Biochemical journal, 475(5), 931-957.

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Measuring DAVEI Binding to gp120

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Recombinantly purified YU2 gp120 protein (50 ng) was immobilized on ELISA plates. Plates were blocked overnight with 3% BSA at 16 °C. Varying concentrations of DAVEI derivatives were added to the plate, and the plate was incubated for 2 h followed by two 30 min washes with 1× PBS. Rabbit anti-CVN (Biosyn Inc.; dilution of 1:3000) was added followed by two washes with 1× PBS. The donkey anti-rabbit HRP conjugate (dilution factor of 1:3000; GE Biosciences, GE NA934V) was used as the secondary antibody, which was detected using an OPD solution.

Parajuli B., Acharya K., Yu R., Ngo B., Rashad A.A., Abrams C.F, & Chaiken I.M. (2016). Lytic Inactivation of Human Immunodeficiency Virus by Dual Engagement of gp120 and gp41 Domains in the Virus Env Protein Trimer. Biochemistry, 55(44), 6100-6114.

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Binding Affinity Assay for CVN and CVN-DAVEI-L2-3Trp

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Plates were preincubated with 150 ng of 2G12 (1: 1000 fold dilution; AIDS repository — Cat. No. 1476) for 2 h followed by three PBS-T washes. Varying concentrations of CVN and CVN-DAVEI-L2-3Trp protein (1000–0.001 nM) were added to the plate and incubated for an additional hour followed by three PBS-T washes. Rabbit anti-CVN (Biosyn, Inc.; dilution 1: 3000) was added as a primary antibody followed by two washes with 1× PBS-T. The donkey anti-rabbit HRP conjugate (GE Biosciences, GE NA934V; dilution factor 1: 3000) was used as the secondary antibody, which was detected using an OPD solution. Plates were developed for 30 min in the dark and the final absorbance was measured at 450 nm using an Infinite m50 (Tecan) plate reader. 2G12 binding was also detected using anti-human HRP (dilution factor 1: 3000; Millipore — AP101P) to ensure it is not lost during the wash process (data not shown).

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Evaluating DAVEI Derivatives Against BaL.01 Viruses

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BaL.01 viruses were diluted (1:20) in 1× PBS and coated onto 48-well ELISA plates, sealed with parafilm, and kept at 4 °C overnight. Plates were blocked the following day with 200 μL of 3% BSA in 1× PBS for 2 h at room temperature. Blocking buffer was discarded (by pipetting), and DAVEI derivatives were added to the viruses at varying concentrations and incubated for 30 min. Plates were washed twice with 1× PBS, 10 min each. Viruses were fixed onto the ELISA plates with 100 μL of 1% PFA (paraformaldehyde) for <15 min. Plates were washed twice with 1× PBS followed by addition of rabbit anti-CVN (Biosyn Inc.; 1:3000 dilution) as the primary antibody, which was detected using the donkey anti-rabbit HRP conjugate (dilution factor of 1:3000; GE Biosciences, GE NA934V) as the secondary antibody. Plates were washed and detected using an OPD solution. Monomeric gp120 (50 ng) directly immobilized onto an ELISA plate was taken as a positive control, and viruses devoid of BaL.01 trimers produced with envelope-deficient pNL4-3 Luc⁺ Env⁻ (8 μg) were used as a negative control for the experiment.

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