Tumour masses were harvested from mice, dissociated with a Gentlemacs instrument (Miltenyi), and digested in DMEM medium containing 1 mg/ml collagenase IA (C2674, Sigma) and 0.02 mg/ml DNAse (D4513, Sigma) at 37°C for 30 min (program 37C_m_LPDK), then filtered with 70‐mm cell strainers (Becton and Dickinson), centrifuged and resuspended in PBS. Resuspended cells were counted by cytometry. The cells were stained with fluorescence‐labelled antibodies in buffer (PBS with 1% BSA, 5 mM EDTA, 0.01% NaN3): FITC‐anti‐Ly6G (#127605, Biolegend), PE‐anti‐CD3 (#553064, BD), PE‐Cy7‐anti‐CD11b (#101215, Biolegend), APC‐Fire750‐anti‐Epcam (#118230, Biolegend), V450‐anti‐CD45.2 (#560697, BD), BV650‐anti‐CD45R‐B220 (#103241, Biolegend), PE‐Dazzle594‐anti‐CD31 (#102429, Biolegend) and Zombie Yellow (#423103, Biolegend), acquisition was performed on the SONY ID7000 (SONY) and analysed using Sony software.
Pe dazzle 594anti cd31
Manufactured by BioLegend
Sourced in United States
PE/Dazzle 594 anti-CD31 is a fluorochrome-conjugated antibody that binds to the CD31 (PECAM-1) cell surface antigen. CD31 is expressed on endothelial cells, platelets, monocytes, and neutrophils. This antibody can be used for the identification and enumeration of cells expressing CD31 in flow cytometric applications.
Automatically generated - may contain errors
Lab products found in correlation
L23105, by Thermo Fisher Scientific (2 mentions)
Brilliant violet 510, by BioLegend (2 mentions)
Brilliant violet785 anti fcgr4, by BioLegend (2 mentions)
Facsaria iiu, by BD (2 mentions)
Lsrii hts, by BD (2 mentions)
Anti cd3 pe, by BD (1 mentions)
Anti cd45 v450, by BD (1 mentions)
Anti cd11b pe cy7, by BioLegend (1 mentions)
Anti ly6g fitc, by BioLegend (1 mentions)
Dnase, by Merck Group (1 mentions)
70 mm cell strainer, by BD (1 mentions)
Id7000, by Sony (1 mentions)
Gentlemacs, by Miltenyi Biotec (1 mentions)
Zombie yellow, by BioLegend (1 mentions)
3 protocols using pe dazzle 594anti cd31
1
Dissociation and Analysis of Tumor-Derived Cells
2
Single-cell RNA-seq Immune Cell Isolation
3
Single-cell RNA-seq Immune Cell Isolation
Check if the same lab product or an alternative is used in the 5 most similar protocols
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Single-cell suspensions were added a live/dead cell staining with blue
reactive dye (1:250, L23105; Invitrogen (Thermo Fisher Scientific, Carlsbad,
USA) and Brilliant Violet 510 anti-CD45 antibody (1:100, 103137; Biolegend, San
Diego, USA) and isolated using the BD FACS Aria Iiu (BD Biosciences, San Jose,
USA). During cell sorting, cellular debris were excluded with FSC and SSC gating
and dead cells excluded with UVB channel negative selection. CD45+cells were then positively selected and purified and processed for single-cell
RNA sequencing as described in [25 (link)].
Flow-cytometric analysis was performed using the BD LSRII HTS (BD Biosciences,
San Jose, USA). In addition to the live/dead and CD45 staining, Brilliant Violet
785 anti-Fcgr4 (1:100, 149535; Biolegend, San Diego, USA) and PE/Dazzle 594
anti-CD31 (1:100, 102429; Biolegend, San Diego, USA) antibodies were used. Data
was analyzed using Flowjo version 10.4.2.
reactive dye (1:250, L23105; Invitrogen (Thermo Fisher Scientific, Carlsbad,
USA) and Brilliant Violet 510 anti-CD45 antibody (1:100, 103137; Biolegend, San
Diego, USA) and isolated using the BD FACS Aria Iiu (BD Biosciences, San Jose,
USA). During cell sorting, cellular debris were excluded with FSC and SSC gating
and dead cells excluded with UVB channel negative selection. CD45+cells were then positively selected and purified and processed for single-cell
RNA sequencing as described in [25 (link)].
Flow-cytometric analysis was performed using the BD LSRII HTS (BD Biosciences,
San Jose, USA). In addition to the live/dead and CD45 staining, Brilliant Violet
785 anti-Fcgr4 (1:100, 149535; Biolegend, San Diego, USA) and PE/Dazzle 594
anti-CD31 (1:100, 102429; Biolegend, San Diego, USA) antibodies were used. Data
was analyzed using Flowjo version 10.4.2.
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