Dehydroepiandrosterone d5 3 sulfate dhea s d5

Manufactured by Merck Group

Dehydroepiandrosterone-d5-3-sulfate (DHEA-S-d5) is a deuterated internal standard used for the quantitative analysis of DHEA-S in biological samples by mass spectrometry techniques.

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Lab products found in correlation

Glucose 6 phosphate dehydrogenase, by Merck Group (2 mentions) 16α hydroxydhea s, by Steraloids (2 mentions) Glucose 6 phosphate (g6p), by Merck Group (2 mentions) β nicotinamide adenine dinucleotide phosphate nadp, by Merck Group (2 mentions) Ritonavir, by LGC (1 mentions) Lopinavir, by LGC (1 mentions) Dhea s standard, by Cayman Chemical (1 mentions) Cyp3a4, by Corning (1 mentions) Dhea s, by Cayman Chemical (1 mentions)

Spelling variants of this product

Dehydroepiandrosterone (14 citations) Dehydroepiandrosterone (DHEA) (10 citations) DHEAS (10 citations) DHEA-d5 (2 citations)

2 protocols using dehydroepiandrosterone d5 3 sulfate dhea s d5

DHEA-S Quantification Using Enzyme Assay

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The DHEA-S standard was purchased from Cayman
Chemical (Ann Harbor, MI). The metabolite standard, 16α-hydroxy
DHEA-S, was purchased from Steraloids (Newport, RI). Glucose-6-phosphate,
Glucose-6-phosphate dehydrogenase, β-nicotinamide adenine dinucleotide
phosphate (NADP⁺), and the internal standard, dehydroepiandrosterone-d₅-3-sulfate (DHEA-S-d₅), were obtained from Sigma-Aldrich (St. Louis, MO). Lopinavir, ritonavir,
and saquinavir mesylate were purchased from Toronto Research Chemicals
(North York, ON, Canada). All other chemicals and solvents used were
obtained from standard suppliers and were of reagent or analytical
grade. The CYP3A4 (catalog number: 456202) and CYP3A7 Supersomes (catalog
number: 456237) were purchased from Corning (Corning, NY) where the
recombinant CYP3A enzymes were coexpressed with the human cytochrome
P450 reductase and the human cytochrome b5 in Sf9 insect cells using
a baculovirus expression system.

Kandel S.E, & Lampe J.N. (2021). Inhibition of CYP3A7 DHEA-S Oxidation by Lopinavir and Ritonavir: An Alternative Mechanism for Adrenal Impairment in HIV Antiretroviral-Treated Neonates. Chemical Research in Toxicology, 34(4), 1150-1160.

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PFAS Impact on CYP3A7 Metabolism

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Dimethyl sulfoxide (DMSO) was purchased from Alfa Aesar (Haverhill, MA). PFOA (CAS: 335–95-5) was purchased from Thermo Fisher Scientific Inc. (Waltham, MA). PFOS (CAS: 2795–39-3) was supplied by Sigma-Aldrich (St Louis, MO). GenX (CAS: 62037–80-3) was purchased from Manchester Organics (Runcorn, UK). PFNA (CAS: 375–95-1) and PFHxS (CAS: 3871–99-6) were both acquired through Frontier Scientific Inc. (Logan, UT). PFNS (CAS: 98789–57-2) was purchased from Toronto Research Chemicals (Toronto, ON). All PFAS stocks were prepared fresh in DMSO prior to each experiment (5–100 mM). Glucose-6-phosphate, Glucose-6-phosphate dehydrogenase, β-nicotinamide adenine dinucleotide phosphate (NADP⁺), and dehydroepiandrosterone-d₅-3-sulfate (DHEA-S-d5) were all purchased from Sigma Aldrich (St. Louis, MO). The fluorescent probe substrate, dibenzylfluorescein (DBF, CAS: 97744–44-0) and DHEA-S (CAS: 1099–87-2) were supplied by Cayman Chemical Company (Ann Arbor, MI). The metabolite standard, 16α-hydroxy DHEA-S, was purchased from Steraloids (Newport, RI). The CYP3A7 Bactosomes (catalog number: CYP/EZ060) were purchased from Cypex Ltd. (Scotland, UK) where the recombinant CYP3A7 enzyme was co-expressed with the human cytochrome P450 reductase in E. coli and then supplemented with the human cytochrome b₅.

Hvizdak M., Kandel S.E., Work H.M., Gracey E.G., McCullough R.L, & Lampe J.N. (2023). Per- and polyfluoroalkyl substances (PFAS) inhibit cytochrome P450 CYP3A7 through direct coordination to the heme iron and water displacement. Journal of inorganic biochemistry, 240, 112120.

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