Abs specific for Flag (D-8), GST(B-14), V5 (E10), p22phox (FL-195), gp91phox (H-60), p47phox (H-195), p67phox (H-300), HK1 (G-1), HK2 (B-8), HK3 (A-9), and Actin (I-19) were purchased from Santa Cruz Biotechnology (Dallas, TX, USA). Specific antibodies against phospho-p47phox (S304, ABIN1526728), (S345, ABIN482777), (S359, ABIN482335), and (S370, ABIN1989372) were purchased from St John’s Laboratory. The antibodies to IRF3 (ab68481), His (ab18184), Myc (ab9106), and AU1 (ab3401) were purchased from Abcam (Cambridge, UK). The antibody to TBK1 (E8I3G) and phospho-TBK1(S172) (D52C2) were from Cell Signaling Technology (Danvers, MA, USA) and STING (NBP 2–24683) was from Novus Biologicals (Centennial, CO, USA).
Ab3401
Manufactured by Abcam
Sourced in United Kingdom
Ab3401 is a laboratory equipment product. It is designed to perform a specific function in the laboratory setting. The core function of Ab3401 is to [DESCRIPTION NOT AVAILABLE].
Automatically generated - may contain errors
Lab products found in correlation
Actin 1 19, by Santa Cruz Biotechnology (2 mentions)
Ab16056, by Abcam (1 mentions)
Il 1β af 401 na, by R&D Systems (1 mentions)
Nlrp3 ag 20b 0014, by Adipogen (1 mentions)
Monoclonal β actin, by Merck Group (1 mentions)
Anti nox4 antibody, by Novus Biologicals (1 mentions)
Alexa 488, by Thermo Fisher Scientific (1 mentions)
3 protocols using ab3401
1
Antibody Validation for Oxidative Stress Signaling
2
Investigating NLRP3 Inflammasome Activation
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LPS (L3024), ATP (A1852), Nigericin (N7143), and DSS (D8906) were purchased from Sigma-Aldrich (St. Louis, MO, USA). Abs specific for Flag (D-8), GST(B-14), V5 (E10), NLRP3 (H-66), ASC (N-15), TXNIP (D-2), Tubulin (5F131), Calnexin (AF18), FACL4 (N-18), caspase-1 (14F468, M-20), and Actin (I-19) were purchased from Santa Cruz Biotechnology (Dallas, Texas, USA). The antibody to calreticulin (D3E6) and IL-18 (D2F3B) was from Cell Signaling Technology (Danvers, MA, USA), IL-1β (AF-401-NA) was from R&D Systems and NLRP3 (AG-20B-0014) was from Adipogen (San Diego, CA, USA). The antibodies to COX IV (ab16056) and AU1 (ab3401) were purchased from Abcam (Cambridge, UK).
3
Immunofluorescence Antibody Validation
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The Alexa488 and 568-labeled goat monoclonal anti-mouse and polyclonal anti-rabbit Fabantibodies were obtained from Life Technologies. The monoclonal anti-V5 antibody was purchased from AbD Serotec. The 16G7, IgG1-type monoclonal anti-p22 phox antibody was previously characterized by the authors [8] . The monoclonal anti-AU1 was obtained from Covance (AFC-130P), while the rabbit polyclonal was ordered from Abcam (ab3401). The monoclonal β-actin and all other chemicals were purchased from Sigma-Aldrich unless otherwise stated. Rapamycin was a kind gift of Péter Várnai. The anti-Nox4 antibody was obtained from Novus Biologicals.
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