β actin antibody bm0627

All chemicals were purchased from Sigma–Aldrich, unless otherwise specified. As for 17β-estradiol (E2758), GPER1 agonist G1 (10,008,933), GPER1 antagonist G15 (14,673) and ICI182780 (1,011,269), these were purchased from Cayman. ERK1/2 antibody (4695) and phosphor-ERK1/2 antibody (4370) were purchased from Cell Signaling Technology; Akt antibody (BM4930), phosphor-Akt antibody (BM4838), β-actin antibody (BM0627) were purchased from BOSTER; p38 antibody (sc-7972), phosphor-p38 antibody (sc-7973), JNK antibody (sc-7345), phosphor-JNK antibody (sc-6254), β-casein (sc-166530) and CK-14 (sc-53253) antibody were purchased from Santa Cruz Biotechnology (Santa Cruz, CA, USA); CK-18 antibody (Abcam, Cambridge, MA, USA, ab668).

Cells were grown to log phase and lysed in Dulbecco’s PBS (150 mM NaCl, 10 mM Na₂HPO₄ and 10 mM NaH₂PO₄, pH 7.4) with 1% SDS and the protease inhibitor cocktail for mammalian cells (Sigma-Aldrich). Total protein concentration was determined by the Bradford method using a commercial reagent from Bio-Rad. Cell extracts or purified proteins were electrophoresed in 10% or 15% SDS-polyacrylamide gel electrophoresis (PAGE) gels, transferred to polyvinyl difluoride (PVDF) membrane, and incubated with specific primary antibodies. Monoclonal antibodies (mAbs) LN2B (anti-Uev1) and 4E11 (anti-Ubc13) were from the laboratory stock [10 (link),39 (link)]. Primary antibodies against HA (sc-7392), MMP1 (sc-30069), NF-κB (sc-372), Lamin B (sc-166729), β-tublin (sc-6216), and secondary goat anti-mouse antibody IgG-horseradish peroxidase (HRP) (sc-2005) and goat anti-rabbit IgG-HRP (sc-2004) antibody were from Santa Cruz. The P-IκBα antibody (#2859S) was from Cell Signaling Technology (Whitby, ON, Canada), while an anti-MMP9 antibody (ab38898) was from Abcam (Toronto, ON, Canada) and β-actin antibody (BM0627) was from Boster (Wunah, China).