Rat elisa kits

To determine the oxidized glutathione (GSSH), the frozen rat livers (~30 mg) were homogenized (1:10 w:v ratio) in the extraction buffer (supplied with the kit) and determined calorimetrically (Abbkine, Inc, Wuhan, China, Cat#: KTB1610). For the lipid peroxidation marker 4-Hydroxy-2-Nonenal (4-HNE), the frozen livers (~30 mg) were homogenized (1:20 w/v ratio) using a RIPA buffer containing SDS and determined using a rat ELISA kit (Abcam, Cambridge, UK, Cat#ab238538). For the classic liver fibrosis marker type-I collagen (COL-1), the frozen livers (~30 mg) were homogenized (1:9 w/v ratio) using PBS (0.01 M, pH = 7.4) and measured using specific rat ELISA kits (Novus Biologicals, Centennial, CO, USA, Cat# NBP2-75823). All procedures were performed according to the manufacturer’s instructions.

Liver tissue homogenate (10%) was prepared in 0.05 M phosphate buffer (pH = 7) using a polytron homogenizer at 4 °C. The homogenate was centrifuged at 10,000 × g for 20 min for removing any cell debris. The supernatant was used after that for the determination of hepatic interleukin (IL)-1β, IL-6, and TGF-β1 levels using rat ELISA Kits (catalog # SEA563Ra, SEA079Ra, and SEA124Ra, respectively, Cloud-Clone Corp, USA). Hepatic contents of OCN and tumor necrosis factor-α (TNF-α) were determined using rat ELISA Kits (catalog # NBP2-68153, Novus Biologicals, and catalog # 438206, BioLegend Company, USA, respectively) following the manufacturer’s instructions (Wang et al. 2020 (link)) and (Guo et al. 2018 ).