Cells were lysed in 2.5% SDS, 250mM Tris-HCl, pH 7.4, at 95°C. Gel electrophoresis and immunoblotting were done as previously described (53 (link)). Immunoblots were developed using primary antibodies directed to CABIN1 (Cell Signaling Technology, (D2B9F), pan-Calcineurin A (Cell Signaling Technology, 2614S), calmodulin (Abcam, Cambridge, MA, EP799Y), SIN3A (Abcam, ab3479), MEF2B (Abcam, EPR22193–26) and GAPDH (Santa Cruz Biotechnology, sc-32233, Inc Dallas, TX,) and HRP-conjugated secondary antibodies. Immunoprecipitations were performed as previously reported (53 (link)) using indicated antibodies.
D2b9f
Manufactured by Cell Signaling Technology
The D2B9F is a laboratory equipment product from Cell Signaling Technology. It serves as a core function, but a detailed description while maintaining an unbiased and factual approach is not available.
Automatically generated - may contain errors
2 protocols using d2b9f
1
Immunoblotting Analysis of Calcineurin Complexes
2
Immunoblotting and Immunoprecipitation Protocol
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Cells were lysed in 2.5% SDS, 250 mmol/L Tris-HCl, pH 7.4, at 95°C. Gel electrophoresis and immunoblotting were done as previously described (53 (link)). Immunoblots were developed using primary antibodies directed to CABIN1 [Cell Signaling Technology (D2B9F), pan-Calcineurin A (Cell Signaling Technology, 2614S), calmodulin (Abcam, EP799Y), SIN3A (Abcam, ab3479), MEF2B (Abcam, EPR22193–26), and GAPDH (Santa Cruz Biotechnology, sc-32233, Inc)] and horseradish peroxidase–conjugated secondary antibodies. Immunoprecipitations were performed as previously reported (53 (link)) using indicated antibodies.
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