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Cd49d bv421 9f10

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CD49d BV421 (9F10) is a fluorescently-labeled antibody that binds to the CD49d protein. CD49d is a cell surface antigen expressed on various cell types. This product can be used for the identification and analysis of cells expressing CD49d in flow cytometry applications.

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Lab products found in correlation

Cd235a pe gpa ga r2, by BD (2 mentions) Hcd45 v450 hi30, by BD (2 mentions) Cd34 fitc 8g12, by BD (2 mentions) Cd71 pe cy7 okt9, by Thermo Fisher Scientific (2 mentions)

Close spelling variants of this product

CD49d (9F10, CD49d-BV421

2 protocols using cd49d bv421 9f10

1

Erythrocyte Differentiation of Edited HSPCs

Check if the same lab product or an alternative is used in the 5 most similar protocols
HSPC were differentiated as described above and then were harvested at day 14-16 for analyses of erythrocyte lineage specific markers. Cells were spun down and washed and then erythrocyte differentiation of edited (WT and HiFi Cas9) and non-edited HSPCs was assessed by flow cytometry using the following antibodies: hCD45 V450 (HI30, BD Biosciences), CD34 FITC (8G12, BD Biosciences), CD71 PE-Cy7 (OKT9, Affymetrix), and CD235a PE (GPA) (GA-R2, BD Biosciences). Reticulocyte differentiation was analyzed using the following antibodies: CD235a PE (GPA) (GA-R2, BD Biosciences), CD49d BV421 (9F10, BD Biosciences), and Band3 APC (a kind gift from Dr. Anumpama Narla and Dr. Mohan Narla).
Vakulskas C.A., Dever D.P., Rettig G.R., Turk R., Jacobi A.M., Collingwood M.A., Bode N.M., McNeill M.S., Yan S., Camarena J., Lee C.M., Park S.H., Wiebking V., Bak R.O., Gomez-Ospina N., Pavel-Dinu M., Sun W., Bao G., Porteus M.H, & Behlke M.A. (2018). A high-fidelity Cas9 mutant delivered as a ribonucleoprotein complex enables efficient gene editing in human haematopoietic stem and progenitor cells. Nature medicine, 24(8), 1216-1224.
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2

Erythrocyte Differentiation of Edited HSPCs

Check if the same lab product or an alternative is used in the 5 most similar protocols
HSPC were differentiated as described above and then were harvested at day 14-16 for analyses of erythrocyte lineage specific markers. Cells were spun down and washed and then erythrocyte differentiation of edited (WT and HiFi Cas9) and non-edited HSPCs was assessed by flow cytometry using the following antibodies: hCD45 V450 (HI30, BD Biosciences), CD34 FITC (8G12, BD Biosciences), CD71 PE-Cy7 (OKT9, Affymetrix), and CD235a PE (GPA) (GA-R2, BD Biosciences). Reticulocyte differentiation was analyzed using the following antibodies: CD235a PE (GPA) (GA-R2, BD Biosciences), CD49d BV421 (9F10, BD Biosciences), and Band3 APC (a kind gift from Dr. Anumpama Narla and Dr. Mohan Narla).
Vakulskas C.A., Dever D.P., Rettig G.R., Turk R., Jacobi A.M., Collingwood M.A., Bode N.M., McNeill M.S., Yan S., Camarena J., Lee C.M., Park S.H., Wiebking V., Bak R.O., Gomez-Ospina N., Pavel-Dinu M., Sun W., Bao G., Porteus M.H, & Behlke M.A. (2018). A high-fidelity Cas9 mutant delivered as a ribonucleoprotein complex enables efficient gene editing in human haematopoietic stem and progenitor cells. Nature medicine, 24(8), 1216-1224.
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