Ampicillin trisodium salt

Luria Broth, ampicillin trisodium salt, L-arabinose, chloramphenicol, phenylmethylsulfonyl fluoride (PMSF), 4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid (HEPES), MgSO₄, adenosine triphosphate (ATP), deoxycytidine, and cytidine diphosphate (CDP) were purchased from Sigma-Aldrich. Isopropyl β-D-1-thiogalactopyranoside (IPTG) was purchased from GoldBio. DEAE and Q-Sepharose resins were obtained from GE Healthcare Life Sciences. Ni-NTA Sepharose resin was purchased from Qiagen. The primers for site-directed mutagenesis was obtained from Integrative DNA Technologies (IDT). BL21(DE3) E. coli competent cells were obtained from New England Biolabs (NEB). Tricarbonyl(1,10-phenanthroline)-(4-bromomethylpyridine)rhenium(I) hexafluorophosphate ([Re]-Br) was available from a previous study.^{22 (link)} [5-³H] CDP was purchased from ViTrax. Alkaline phosphatase (AP, calf intestine) was purchased from Roche. Thioredoxin (TR) and thioredoxin reductase (TRR) were available from a prior study.^{20 (link)}

Luria broth, ampicillin trisodium salt, phenylmethylsulfonyl fluoride (PMSF), 4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid (HEPES), MgSO₄, adenosine triphosphate (ATP), deoxycytidine, cytidine diphosphate (CDP) and alkaline phosphatase (AP, calf intestine) were purchased from Sigma-Aldrich. Isopropyl β-D-1-thiogalactopyranoside (IPTG), L-arabinose, kanamycin (Km), chloramphenicol (Cm), dithiothreitol (DTT) were purchased from GoldBio. DEAE and Q-sepharose resins were obtained from GE Healthcare Life Sciences. Ni-NTA agarose resin was purchased from Qiagen. PD-10 desalting columns packed with Sephadex G-25 resin (10 mL) were obtained from Cytiva. The primers for site-directed mutagenesis were obtained from Integrative DNA Technologies (IDT). E. coli BL21(DE3) competent cells were obtained from New England Biolabs (NEB). [5-³H]-CDP was purchased from ViTrax. Emulsifier-Safe scintillation cocktail was obtained from Perkin-Elmer. 2,3,5-Trifluorotyrosine (2,3,5-F₃Y_xxx, where xxx indicates the residue number) and 3,5-difluorotyrosine (3,5-F₂Y_xxx, where xxx indicates the residue number), thioredoxin (TR) and thioredoxin reductase (TRR) were available from a prior study. Tricarbonyl(1,10-phenanthroline)(4-bromomethylpyridine) rhenium(I) hexafluorophosphate ([Re]-Br) was synthesized via the previously modified procedure.^{22 (link)}