TNF-α and IL-1β expression levels from OA model and Control rabbit synovial fluid or condition medium of human synovial fibroblasts following cell treatment and LPS simulation were detected using the following ELISA kits purchased from R&D Systems, Inc. (Minneapolis, MN, USA): Rabbit TNF-α kit (cat. no. DY5670), human TNF-α kit (cat. no. DY210-05), rabbit IL-1β kit (cat. no. DY7464) and human IL-1β kit (cat. no. DY201-05). Following this, absorbance values were measured at 450 nm to determine the concentrations, according to the manufacturer's protocol.
Rabbit tnf α kit
Manufactured by R&D Systems
Sourced in United States
The Rabbit TNF-α kit is a diagnostic tool designed to quantitatively measure Tumor Necrosis Factor alpha (TNF-α) levels in rabbit samples. It utilizes the Enzyme-Linked Immunosorbent Assay (ELISA) technique to provide accurate and reliable results.
Automatically generated - may contain errors
2 protocols using rabbit tnf α kit
1
Quantifying Inflammatory Cytokines in OA and Healthy Synovium
2
Rabbit Model of Endotoxin-Induced Inflammation
Check if the same lab product or an alternative is used in the 5 most similar protocols
(1) Animals. A total of 30 healthy male New Zealand White (NZW) rabbits, weighing 2–2.2 kg, were provided by the Experimental Animal Center of Shanghai University of Traditional Chinese Medicine [license number: SXCK (Shanghai) 2012-0008]. Basal body temperature (BBT) screening was conducted prior to the experiment.
(2) Major Agent. Bacterial endotoxin (Sigma, L2630, 10 mg), type I collagenase (Sigma, lot number: C0130), Masson staining kit (Beijing Reagan, lot number: DC0032), rabbit TNF-α kit (R&D Systems subpackage, rb201510200842), IL-1β kit (R&D Systems subpackage, rb201510190821), and IL-4 kit (R&D Systems subpackage, rb201510270951) were the major agents used in this study.
(3) Major Instruments. Pyrogen measurer (ZRY-3, Tianjin Tianda Tianfa), Upright Microscope (CX41, OLYMPUS), IMS Image Analysis System (Jrdun Biotechnology, Shanghai), Microplate Reader, and a spectrophotometer were used in this study.
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(1) Animals. A total of 30 healthy male New Zealand White (NZW) rabbits, weighing 2–2.2 kg, were provided by the Experimental Animal Center of Shanghai University of Traditional Chinese Medicine [license number: SXCK (Shanghai) 2012-0008]. Basal body temperature (BBT) screening was conducted prior to the experiment.
(2) Major Agent. Bacterial endotoxin (Sigma, L2630, 10 mg), type I collagenase (Sigma, lot number: C0130), Masson staining kit (Beijing Reagan, lot number: DC0032), rabbit TNF-α kit (R&D Systems subpackage, rb201510200842), IL-1β kit (R&D Systems subpackage, rb201510190821), and IL-4 kit (R&D Systems subpackage, rb201510270951) were the major agents used in this study.
(3) Major Instruments. Pyrogen measurer (ZRY-3, Tianjin Tianda Tianfa), Upright Microscope (CX41, OLYMPUS), IMS Image Analysis System (Jrdun Biotechnology, Shanghai), Microplate Reader, and a spectrophotometer were used in this study.
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