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Rb 1197 r7

Manufactured by Thermo Fisher Scientific
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The RB-1197-R7 is a laboratory centrifuge designed for general-purpose applications. It has a maximum speed of 6,000 rpm and can accommodate a variety of sample tubes and rotors. The centrifuge is suitable for separating materials of different densities.

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Lab products found in correlation

Ab194726, by Abcam (2 mentions) Ab205718, by Abcam (2 mentions)

2 protocols using rb 1197 r7

1

Immunohistochemical Analysis of Cochlear Samples

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Another group of cochlear paraffin sections was used for caspase-3 detection by a rabbit polyclonal antibody (cat. no. RB-1197-R7 Thermo Fisher Scientific, Waltham, MA, USA). For the NF-κB investigation, some cochlear paraffin sections were obtained, stained for NF-κB using rabbit polyclonal NF-_B p65 (phospho S276) primary antibody (ab194726), goat anti-rabbit IgG H&L (HRP) secondary antibody (ab205718) (Abcam, Cambridge, UK), and 3,3′-diaminobenzidine chromogen following the ABC technique (Ramos-Vara et al. 2008 (link)).
Abd-Elhakim Y.M., Abdel-Motal S.M., Malhat S.M., Mostafa H.I., Ibrahim W.M., Beheiry R.R., Moselhy A.A, & Said E.N. (2022). Curcumin attenuates gentamicin and sodium salicylate ototoxic effects by modulating the nuclear factor-kappaB and apoptotic pathways in rats. Environmental Science and Pollution Research International, 29(60), 89954-89968.
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2

Immunohistochemical Detection of Apoptosis and NF-κB

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Another set of kidney paraffin sections was used for Caspase-3 detection by a rabbit polyclonal antibody (cat no: RB-1197-R7 Thermo Fisher Scientific, Waltham, MA, USA). For NF-κB investigation, some kidney paraffin sections were used, stained for NF-κB by rabbit polyclonal NF-κB p65 (phospho S276) primary antibody (ab194726), goat anti-rabbit IgG H&L (HRP) secondary antibody (ab205718) (Abcam, Cambridge, UK), and 3,3′-diaminobenzidine chromogen in line with the ABC method [54 (link)]
Abd-Elhakim Y.M., Moselhy A.A., Aldhahrani A., Beheiry R.R., Mohamed W.A., Soliman M.M., Saffaf B.A, & M. El Deib M. (2021). Protective Effect of Curcumin against Sodium Salicylate-Induced Oxidative Kidney Damage, Nuclear Factor-Kappa Dysregulation, and Apoptotic Consequences in Rats. Antioxidants, 10(6), 826.
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