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Anti pd 1 apc clone eh12.2h7

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Anti-PD-1-APC (clone EH12.2H7) is a fluorochrome-conjugated antibody that binds to the PD-1 protein. PD-1 is a cell surface receptor expressed on activated T cells, B cells, and myeloid cells. This product can be used for flow cytometric detection and analysis of PD-1 expression.

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Lab products found in correlation

Facscanto 2, by BD (1 mentions) Anti cd8 fitc clone t8, by Beckman Coulter (1 mentions) Dextramer rpe, by Immudex (1 mentions) Clone okt3, by Thermo Fisher Scientific (1 mentions) Anti cd3, by Thermo Fisher Scientific (1 mentions) Anti cd69 apc clone fn50, by BioLegend (1 mentions)

Spelling variants of this product

PD-1 (EH12.2H7, (20 citations) Anti-PD-1-APC (18 citations) EH12.2H7 (13 citations) Anti-PD-1 (clone EH12.2H7, (9 citations) PD-1 (clone EH12.2H7), (9 citations) Anti-PD-1 (EH12.2H7, (7 citations) Clone EH12.2H7 (5 citations) PD-1 (EH12.1) (4 citations) APC-PD-1 (4 citations) PD-1 APC (clone EH12.2H7; (2 citations)

2 protocols using anti pd 1 apc clone eh12.2h7

1

PBMCs Dextramer Staining and Flow Cytometry

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PBMCs were stained using HLA-A*02:01 Dextramer-RPE [GPC3144–152 (FVGEFFTDV), HIV19–27 (TLNAWVKVV) or negative control; Immudex] and HLA-A*24:02 Dextramer-RPE [GPC3298–306 (EYILSLEEL), HIV583–591 (RYLKDQQLL); Immudex] for 15 min at room temperature, followed by anti-CD8-FITC (clone T8, Beckman Coulter), anti-PD-1-APC (clone EH12.2H7, BioLegend), or isotype control-APC (clone MOPC-21, BioLegend) for 20 min at 4°C. Flow cytometry was performed using a FACSCanto II (BD Biosciences).
SAWADA Y., YOSHIKAWA T., SHIMOMURA M., IWAMA T., ENDO I, & NAKATSURA T. (2014). Programmed death-1 blockade enhances the antitumor effects of peptide vaccine-induced peptide-specific cytotoxic T lymphocytes. International Journal of Oncology, 46(1), 28-36.
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2

PBMC Activation and Phenotyping

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Blood samples were drawn at several time points during treatment. Peripheral blood mononuclear cells (PBMCs) were purified, washed and counted. 1 × 105 Lymphocytes in each sample were activated with plate bound anti CD3 (1.0 μg/ml, clone OKT3, Invitrogen). The cells were than stained with the following antibodies: anti- CD8 PB (clone RPA-T8, BioLegend), anti PD-1 APC (clone EH12.2H7, BioLegend), anti CD69 APC (clone FN50, BioLegend).
Hadash-Bengad R., Hajaj E., Klein S., Merims S., Frank S., Eisenberg G., Yakobson A., Orevi M., Caplan N., Peretz T., Lotem M, & Cohen J.E. (2020). Immunotherapy Potentiates the Effect of Chemotherapy in Metastatic Melanoma—A Retrospective Study. Frontiers in Oncology, 10, 70.
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